Total Phenols, Antioxidant Capacity and Antibacterial Activity of Manuka Honey Extract
Journal Title: Journal of Advances in Biology & Biotechnology - Year 2017, Vol 15, Issue 4
Abstract
Aims: To evaluate total phenols content (TPC), antioxidant capacity (TAC) and antibacterial activity of Manuka honey extract (MHE) and to compare such properties with those for unfractionated Manuka honey. Study Design: In vitro study. Place and Duration of Study: School of Biomedical Sciences, Ulster University, Coleraine, UK. Between September 2016 and September 2017. Methodology: MHE was prepared by solvent extraction using ethyl acetate. TPC was determined by Folin-Ciocalteu assay. The iron (III) reducing antioxidant capacity (IRAC) method was used to determine TAC. Antibacterial activity was evaluated using disc diffusion assay and 96-well microtiter plate methods with absorbance measured at 600 nm. Results: The TPC for MHE was 30-fold higher than the value for Manuka honey (33420 ± 1685 mg vs. 1018 ± 78 mg GAE/kg) while TAC values were ~100-times greater (83,198 ± 7064 vs. 793 ± 104 TEAC, respectively). Antibacterial activity assessed by disc diffusion for Manuka honey (18.5mm on S. aureus and 20 mm on E. coli) was two times greater than for MHE (9mm for both S. aureus and E. coli). The 96-well microtiter plate assay confirmed the greater antibacterial activity for Manuka honey compared to equal concentrations MHE. Conclusion: A polyphenol-rich Manuka honey extract with a high total antioxidant capacity, showed little or no antibacterial activity against E. coli and S. aureus in contrast with unfractionated Manuka honey.
Authors and Affiliations
Tsz Ching Chau, Richard Owusu-Apenten, Poonam Singh Nigam
Keywords
Related Articles
- EP ID EP318146
- DOI 10.9734/JABB/2017/37101
- Views 98
- Downloads 0
A Morphometric Study on Two Groups of Brown Trout (Salmo trutta) Population, 1758 in Northern Region of Albania
This study was carried out to determine morphological differences between two brown trout (Salmo trutta Fario) populations of two Alpine rivers in Northern Albania: Cem River and Valbona River. Fifty five single individu...
Comparative Assessment of the Impact of Organic and Inorganic Fertilizers Application on the Growth and Development of Solanum nigrum L. (Angiosperm; Solanaceae)
Aims: The application of organic and inorganic fertilizers to the soil is considered a good agricultural practice as it helps to improve the fertility of the soil and the quality of plant products. However, the effect of...
Hemophilia A and Induced Pluripotent Stem Cells
Hemophilia A is a hemorrhage disorder inherited according to the X-linked inheritance pattern. It affects about 1 in 4,000 to 10,000 males. Permanent changes in the F8 gene result in hemophilia A. These changes result in...
The Amelioration of Cyanide Induced Liver Toxicity with Bentonite Using Wistar Rat as Experimental Model
Aim: The objective of this study was to investigate the ameliorating effect of a natural bentonite found in Nigeria on liver enzymes of Wistar rat fed with cyanide from cassava waste water. The enzymes assayed were alkal...
Evaluation of α-Amylase Inhibitory Potentials of Sida acuta, Tithonia diversifolia and Chromolaena odorata Leaf Extracts
Aim: To investigate the inhibitory effect of Sida acuta, Tithonia diversifolia and Chromolaena odorata leaf extracts on the activity of carbohydrate hydrolyzing enzyme (α-amylase) and to provide scientific validation of...