TP53 Gene Alterations and P53 Protein Accumulation in Human Esophageal Cancer in Khartoum State, Sudan.
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2018, Vol 17, Issue 11
Abstract
TP53 gene alterations were screened in 50 formalin fixed paraffin embedded (FFPE) human esophageal cancer (EC) specimens comprising 43 squamous cell carcinoma (SCC) and 7 adenocarcinoma (AC) using immunohistochemistry (IHC) and conventional PCR followed by DNA sequencing to detect p53 accumulation and mutation, respectively, in Khartoum state, Sudan. TP53 gene mutations were detected in 20 (40%) of the 50 EC cases investigated. Five out of 13 (38.5%) of SCC cases that stained positive for p53 protein, showed TP53 mutations, only one (14.3%) of seven AC samples was positive for p53 protein without associated mutation and 4/7 (57.1%) showed TP53 mutations only. Immunohistochemical stain was positive in 14/50 (28%) of EC samples; 13 (92.9%) of them were SSC cases and one (7.1%) AC. Five out of forty three (11.6%) of SSC and none of AC cases showed both immunohistochemical positive stain and mutation of TP53 gene while 8/43 (18.6%) of SSC and 1/7 (14.3%) of AC samples showed immunohistochemical positive stain but no TP53 gene mutation were present. Finally 15/50 (30%) of EC, 11 (73.3%) were SSC and 4 (26.7 %) AC showed immunohistochemical negative stain and positive TP53 gene mutations. Immunohistochemical patterns of p53 were not related significantly to mutational analysis results in the cases examined. Mutation analysis is recommended to be done on the entire length of the gene using fresh tissue to complete the whole picture in future studies.
Authors and Affiliations
Rihab M. Elfaki, Mohammed S. AbdelAziz, Hisham N. Eltayb, Munsoor M. Munsoor, Ahmed A. Gameel
Keywords
Related Articles
- EP ID EP416496
- DOI 10.9790/0853-1711086267.
- Views 72
- Downloads 0
A Morphometric Study of Dry Human Typical Thoracic Vertebral Body in Telangana Region.
Background: Previous studies in the past about morphometry of thoracic vertebrae mainly focused on pedicle diameters and their angulations. The vertebral body were not studied particularly in Telangana Region that is the...
Strategic Bidding Issues in Power System: An Empirical State-ofthe-Art Developments
Strategic bidding issues in electricity markets are extensively investigated in power systems, often by modeling multifaceted bi-level optimization issues, which are hard enough to resolve. The conventional approach for...
Finger Print Ridge Density a Tool for Gender Determination
Background: Fingerprints being unique to a human being, is a valuable tool in identification. Identification of sex from fingerprint ridge density has been attempted by earlier researchers. Objectives: The objective of t...
A trend of early epiphyseal fusion in Bengali female as compared to Galstaun's data
The present study is conducted to assess the applicability of age old Galstaun’s data to determine age from closure of secondary centers of ossification in Bengali population. Radiographs of elbow & wrist joints are take...
Functional Outcome of Acromioclavicular Dislocation Treated By Hook Plate
Background: Internal fixation with hook plate has been used to treat acromioclavicular joint dislocation. This study aims to evaluate the effect of its use on shoulder function, to further analyze the contributing factor...