TRYPANOCIDAL, ANTI-LEISHMANIAL, AND CYTOTOXIC ACTIVITY OF MUEHLENBECKIA TAMNIFOLIA (KUNTH) MEINS (POLYGONACEAE)
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2019, Vol 12, Issue 7
Abstract
Objective: The objective of this study was to evaluate the activity against amastigotes of Leishmania panamensis and epimastigotes of Trypanosoma cruzi (Chagas), extracts and fractions obtained from leaves and stems of Muehlenbeckia tamnifolia. Methods: Plant material was collected during the flowering, in the town of La Calera (Cundinamarca), at a height of 2746 m above sea level, 4°43’11”N, 3°58’12”W. Leaves and stems were extracted with light petroleum and then with ethanol. The extracts were fractionated by column chromatography on silica gel with petrol; CH2Cl2, AcOEt, and MeOH. The activity against epimastigotes and cytotoxicity was evaluated by the enzymatic micromethod with MTT. The active extracts against epimastigotes and with low cytotoxicity were also evaluated in trypomastigotes and intracellular amastigotes. Results: The dichloromethane fraction from leaves and stems of M. tamnifolia showed the highest activity against Leishmania panamensis with an 50% of the effective concentration of 0.006 (μg/ml) and a selectivity index of 4.16. In U937 cells, six of the extracts and fractions tested showed high cytotoxicity, 50 inhibitory concentration <50 μg/ml. Conclusions: The extracts obtained from leaves and stems of different polarities of M. tamnifolia (Kunth) Meins, revealed a moderate effect against amastigotes of L. panamensis (Leishmaniosis) (low polarity fractions) and a low effect against epimastigotes of T. cruzi (Chagas).
Authors and Affiliations
RODRÍGUEZ A OSCAR E , TORRENEGRA G RUBÉN D , POMBO O LUIS M
Keywords
Related Articles
- EP ID EP635662
- DOI -
- Views 138
- Downloads 0
ANTIHYPERTENSIVE EFFECT OF RUTIN: PHARMACOLOGICAL AND COMPUTATIONAL APPROACH
Objective: Phenolic compounds, such as flavonoids, have aroused great scientific interest due to their diverse pharmacological activities. Several studies suggested that flavonoids act as antihypertensive by inhibiting a...
DESIGN, SYNTHESIS, MOLECULAR DOCKING, AND BIOLOGICAL EVALUATION OF PYRAZOLE 1-CARBOTHIAMIDE INCORPORATED ISOXAZOLE DERIVATIVES
Objectives: Novel isoxazole incorporated pyrazole carbothiamide 5 (a-r) was designed and synthesized, docked and evaluated for anticancer activity Michigan Cancer Foundation-7 (MCF-7), and breast cancer cell lines. Mate...
EFFECT OF SUPPLEMENTATION OF WITHANIA COAGULANS FRUIT ON THE FASTING PLASMA GLUCOSE LEVELS OF TYPE 2 DIABETIC SUBJECTS
Objective: The objective of this study is to determine the effect of supplementation of Withania coagulans fruit on the fasting plasma glucose (FPG) levels of type 2 diabetic subjects.Methods: In the natural system of me...
STUDY ON APHRODISIAC ACTIVITY OF OLEA DIOICA ROXB. BARK, LEAF EXTRACTS, AND ITS PURE COMPOUND USING WISTAR ALBINO RATS
Objectives: We aimed to assess the effects of O. dioica Roxb. leaf and bark extract and its isolated constituent benzeneethanol, 4-hydroxy- on aphrodisiac activity in rats using different standard aphrodisiac parameters....
FACILITATORS AND BARRIERS FOR BEDSIDE TEACHING IN THE TEACHING HOSPITALS OF COASTAL SOUTH INDIA
 Objectives: The aim was to assess and compare the perception of clinical faculty toward bedside teaching (BST) for undergraduate medical studentsand the barriers encountered by them.Methods: The present cross-sectional...