Tumor necrosis factor-α-activated mesenchymal stem cells accelerate wound healing through vascular endothelial growth factor regulation in rats
Journal Title: Universa Medicina - Year 2018, Vol 37, Issue 2
Abstract
Background Wounds are areas of physical or thermal damage of the epithelial layer of skin or mucosa. The wound healing process consists of hemostasis, inflammation, proliferation, and remodeling. Mesenchymal stem cells (MSCs) play a role in wound healing by suppressing potent pro-inflammatory molecules, such as tumor necrosis factor-α (TNF-α), leading to macrophage polarization from the pro-inflammatory type to the pro-regeneration type characterized by increasing vascular endothelial growth factor (VEGF) production. MSCs are able to increase VEGF level in-vivo correlated with collagen synthesis. The objective of this study was to assess the role of TNF-α-activated MSCs on VEGF in rat wounds. Methods An experimental animal study with post-test only control group design was performed involving 24 Wistar rats. The rats were randomized into four groups consisting of one control (K) and three treatment groups (P) (activated MSCs at doses of 3x105, 6x105, and 12x105 cells, respectively). The measurement of VEGF levels was done using ELISA assay while the collagen analysis was performed by light microscopy. One way ANOVA and Post Hoc LSD were used to analyze the data. Results The results showed a significant increase in VEGF levels (p <0.05) on day 3 and then a significant decrease on day 5 along with a significant increase in the amount of collagen on day 7 (p<0.05). Conclusion This study demonstrated that TNF-α-activated MSCs were able to regulate VEGF levels and collagen synthesis in wound healing in rats. The molecular mechanism by which TNF-α-activated MSCs stimulate cutaneous wound healing should be clarified further.
Authors and Affiliations
Aditya Nugraha, Agung Putra
Keywords
Related Articles
- EP ID EP382333
- DOI 10.18051/UnivMed.2018.v37.135-142
- Views 231
- Downloads 0
Consumption of elicited soybean sprout flour increases calcium level in serum of ovariectomized rats
Background The decline in estrogens could lead to an imbalance of bone formation and absorption as well as bone mineralization. Phytoestrogens are known to have the potential to maintain bone health. Calcium and phosphor...
Combination of aerobic exercise and Hibiscus sabdariffa Linn. increased nitric oxide in rats
Background Hypertension and myocardial infarction account for the high rate of mortality globally. Hibiscus sabdariffa (HS) Linn. is rich in antioxidants and previous studies have demonstrated its anti-hypertensive effe...
Habit of cooking pork on hot stones as main risk of cysticercosis
Background Cysticercosis is an infectious disease caused by the larval form of Taenia solium (cysticercus cellulosae) and has been ranked as the most important food-borne parasite of humans in terms of public health, soc...
New tuberculosis vaccine to support tuberculosis elimination
The Sustainable Development Goals have prioritized ending the epidemic of tuberculosis by 2030.(1) Around one-third of the world population is infected with Mycobacterium tuberculosis (MTBC), but is asymptomatic, a condi...
Increase in neutrophil count after repeated exposure of Plasmodium berghei-infected mice to artemisinin
Background Leukocytes play an important role in the elimination of malaria infection. The leukocyte profile upon elimination of the malaria parasites that have been exposed to antimalarials and are subsequently capable o...