Use of stem sections from in vitro papaya plants (hybrid IBP 42-99) to obtain callus with embryogenic structures
Journal Title: Biotecnología Vegetal - Year 2004, Vol 4, Issue 4
Abstract
Inside in vitro propagation via, the somatic embriogenesis offers possibilities of obtaining top volumes of production in a minor period of time and a lower cost, which are a method potentially more efficient than the regeneration via organogenesis. In papaya the somatic embryogenesis could have developed from zigotic embryos and axis hipocotilos, nevertheless in case of the hybrids these methods cannot be used and it becomes necessary to develop it from a somatic fabric, without link with the sexual reproduction. This work chased as main objective Evaluated the use of in vitro plants stem sections of the Carica papaya IBP 42-99 hybrid for the formation of callus with embryogenic structures. As plant material were use in vitro plants of the papaya hybrid IBP 42-99. For it there took sections of different parts of the stem from the meristem up to the base of the in vitro plants, was use the culture medium Nitsh and Nitsh supplemented with 1.5 mg.l-1 of 6-BAP and 1.5 mg.l-1 of AIA. It was achieved to obtain callus from the stem sections with the culture medium used, nevertheless, in the treatments where used cylinders inside 1.0 cm from the apex down, the best results were achieved. The use of in vitro plants stem sections as explant for the formation of callus in this vegetable species it opens new possibilities for his in vitro propagation, specially in case of resultant hybrids of genetic improvement programs.
Authors and Affiliations
Jorge Gallardo Colina, Rafael Gómez Kosky, Marisol Tejeda Fernández, Laisyn Posada Pérez, Idalia Herrera O´farril, Maritza Reyes Vega, Leyanis García Aguila, Marisol Freire Seijo
Keywords
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