Use of Vitrofural in the chemical sterilization of the artificial endosperm of encapsulated somatic embryos of Saccharum spp. hybrid var Cuba 87- 51
Journal Title: Biotecnología Vegetal - Year 2002, Vol 2, Issue 4
Abstract
The effect of the Vitrofural in the control of pollutants in the capsule study was carried out, as well as its toxic effect on the somatic embryos of sugar cane. Different dose of this compound was used (20, 25, 30, 35 and 40 mg l-1), these they were applied to the cultivation medium with nudes somatic embryos, which was used previously autoclaved and without autoclaved, later these same doses were added to the synthetic endosperm. The Vitrofural can be employed in the chemical sterilization of the cultivation medium for the germination in vitro of the somatic embryos, as well as in the chemical protection of the synthetic endosperm, that which allowed to eliminate the autoclaved of the encapsulation medium, as well as to reduce the concentration of the agents gelificantes in 33% and to carry out all the operations under non sterile conditions.
Authors and Affiliations
Elisa Quiala, Elio Jiménez, Manuel de Feria, Yelenys Alvarado, Maité Chávez, Daniel Agramonte, Daymí Ramírez, Mayra Acosta, Nayvi Pérez, Alina Capote
Keywords
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