UV-ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF DAPOXETINE HYDROCHLORIDE AND SILDENAFIL CITRATE IN TABLET DOSAGE FORM
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2019, Vol 12, Issue 1
Abstract
Objective: The objective of this research was to develop and validate a simple ultraviolet (UV) spectrophotometric method for simultaneous determination of sildenafil citrate and dapoxetine hydrochloride in a pharmaceutical formulation.Methods: Two simple UV spectrophotometric methods have been developed for simultaneous determination of sildenafil citrate and dapoxetine hydrochloride. For both methods, stock solutions were prepared in methanol followed by the further required dilutions with methanol. Proposed dual-wavelength method and ratio derivative method, the wavelength of maximum absorption for sildenafil citrate and dapoxetine hydrochloride was 292 nm and 231 nm, respectively.Results: In both methods, the linearity range lies between 10 and 60 μg/ml for sildenafil citrate and 2–12 μg/mL for dapoxetine hydrochloride with their respective wavelengths. By dual-wavelength method, the percentage of sildenafil citrate and dapoxetine hydrochloride was found to be 101.3% and 100.3%, respectively.Conclusion: Result obtained in this research work clearly indicated that both these methods were found to be accurate, precise, stable, and robust as indicated by low values of percentage relative standard deviation. Thus, the present study gives an excellent method for the determination of both the drugs in combined tablet formulation.
Authors and Affiliations
Hemesh Gadiya, Monika Maheshwari, Ashok Dashora
Keywords
Related Articles
- EP ID EP607155
- DOI 10.22159/ajpcr.2019.v12i1.29937
- Views 122
- Downloads 0
A SIMPLE REVERSE PHASE-HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD DEVELOPMENT AND VALIDATION OF VALSARTAN IN BULK AND IT’S TABLET DOSAGE FORM
Objective: The most important objective of the present research work is to develop simple, specific, rapid, accurate, and sensitive reverse-phase high-performance liquid chromatography method and validated for the qualit...
PARTICULATE DELIVERY SYSTEM OF CHITOSAN - DITERPENE LACTONE FRACTION OF SAMBILOTO (ANDROGRAPHIS PANICULATA NEES): PREPARATION, CHARACTERIZATION AND IN VITRO DRUG RELEASE
Objective: This paper was aimed to study the effect of chitosan, sodium tripolyphosphate (TPP) as crosslinker, and diterpene lactone fraction of sambiloto (FDTL) from Andrographis paniculata Nees which contained 75.9% an...
DESIGN AND DEVELOPMENT OF NOVEL DRUG DELIVERY SYSTEM USING HERBAL MEDICINE TO TREAT ALZHEIMER’S DISEASE
Objective: The present study was focused to design an herbal formulation for the treatment of Alzheimer’s disease (AD) to develop the formulation using various techniques such as spray drying, centrifugation, and lyophil...
IN VITRO-IN VIVO CORRELATIONSHIP APPROACH OF THE PREPARED MAGNETIC MICROSPHERES OF CYTARABINE
Objective: The objective of this study was to study the in vitro-in vivo correlationship between the magnetic microspheres prepared by continuous solvent evaporation (CSE) method by 32 factorial designs. Methods: CSE te...
OPTIMUM CONDITIONS FOR N-ACETYL GLUCOSAMINE PRODUCTION FROM TIGER SHRIMP (PENAEUS MONODON) SHELL BY SERRATIA MARCESCENS
Objective: The aim of this research was to determine the optimum condition for Serratia marcescens to produce optimum amount of N-acetyl glucosamine using chitin isolated from tiger shrimp (Penaeus monodon) shells.Method...