Варіабельність морфологічних і біохімічних ознак листків рослин роду Tilia L. в урбосередовищі [Variability of morphological and biochemical features in leaves of genus Tilia L. in urban ecosystem]
Journal Title: Наукові праці Лісівничої академії наук України - Year 2016, Vol 14, Issue
Abstract
In urban ecosystems plants accumulate phytotoxic compounds and stay during long time under physiological stress conditions. Plants of genus Tilia are highly sensitive to stress factors and have morphological plasticity of vegetative organs. The aim of this work is compare analysis of variability morphological features and biochemical profiles of phenolic compounds in leaves of 5 species of genus Tilia. Studies of morphological features and physiological condition had been implementing during 2015-2016 yr. Leaves of generative trees on Saksaganskogo str. (Kyiv) was sampled in first part of June and late August. As a control was used plants from collection of M.M. Gryshko National botanical garden. Phytochemical researches carried out on methanol extracts of leaves Tilia platyphyllos, Tilia begoniifolia, Tilia cordata, Tilia × europaea, Tilia tomentosa. Qualitative analysis of phenolic compounds was investigated by high-performance thin-layer chromatography method using system of solvents chloroform- acetic acid-methanol-water (v/v/v/v - 60/32/12/8). Degree assessment of leaves damages was carried out by Krasinskyi method. Determination of affected surface leaf area was implemented with using software Image Pro Premier 9.1. Reliability of averages differences was defined to least significant difference (LSD) p > 0,05. Correlation, regression and principal component analysis was performed in the software Statistica 7.0. In the article was shown results of variability analysis of morphometric parameters in leaves of five species of genus Tilia L. It was found that leaves area of investigated plants had close connections with width of leaf lamina (r = 0,98). In plants of T. platyphyllos Scop., T. × europea L., T. tomentosа Moench., T. begoniifolia Steven under vehicular pollution conditions was determined that ratio coefficient of lamina perimeter to area square root are increasing. This index is stabile for each species of Tilia. Its deviation from average value is an informative marker of deterioration plants general conditions. Morphological features of leaves genetically connected with phytochemical profiles of phenolic compounds. Their qualitative and quantitative composition allows carry out species identification and determine level of physiological stress and potential resistance of plant organism. Biochemical polymorphism of genus Tilia plants allows to use chromatographic profiles for chemosystematics. In leaves of urban plantations of genus Tilia L. was found increasing of phenolic compounds content in particular cinnamic acids and their conjugates. T. tomentosa was the most stable to certain morphological and biochemical markers under Kyiv conditions. The results of studies show new aspects of solving problem of hybrids origin, genus systematics and plant adaptation for habitats in processes of introduction and acclimatization.
Authors and Affiliations
Nadia Oleksijchenko, Artur Likhanov
Keywords
Related Articles
- EP ID EP192527
- DOI -
- Views 123
- Downloads 0
Методологічні аспекти створення геоінформаційної системи лісових автодоріг [Methodological aspects of geoinformation system development for forest roads]
The article analyzes trends of the geographic information systems (GIS) development in forestry and international requirements to the environmentally friendly technologies on the basis of optimal transport network, as pr...
Стан, структура і тенденції розвитку основних лісових формацій Косівщини [Condition, structure and development trends of the main forest formations in Kosivshchyna]
Forest Fund of Kosivshchyna (Ivano-Frankivsk region) is distinguished by the significant variety of forest- and forest site types, species composition and structure of forest formations which are located within 270-1472...
Norway spruce in the Right Bank Forest-Steppe of Ukraine: perspectives of use and improvement of reproduction methods Ялина європейська у Правобережному Лісостепу України: перспективи використання та вдосконалення способів розмноження
Today, in Ukraine and abroad, the drying up of Norway spruce trees in forest plantations of different purpose has become a serious problem. Considering the important economic and social significance of spruce, studies of...
The influence of plantation-type forests on the water-based and physical properties of forest soil in the conditions of the Western Forest-Steppe of Ukraine Вплив лісових насаджень плантаційного типу на водно-фізичні властивості лісового ґрунту в умовах Західного Лісостепу України
There is a close interaction between stands of different species composition and soil, which can be influenced by changing the composition of the stand. The issue is particularly relevant in terms of the temporary intr...
Stock of forest litter and ground vegetation as an indicator of fire risk in the pine forests of Polissya Запаси підстилки та живого надґрунтового вкриття як показник ризику виникнення пожеж у соснових лісах Полісся
The actual problem for forestry in Ukraine is the forest protection from fires and minimization of losses caused by them. Solving these problems is possible only with the accurate prediction of the forest fires occurrenc...