Viral metagenomics analysis of poultry faeces in live poultry market, Haikou, China
Journal Title: Asian Pacific Journal of Tropical Medicine - Year 2018, Vol 11, Issue 10
Abstract
Objective: To conduct in-depth study of the distribution and diversity of viruses in poultry is of great importance in monitoring the emergence of interspecies transmission of novel viruses that may cause epidemics with public health significance. Poultry is an economically important source of meat, eggs and feathers which plays an important role as natural reservoirs of many pathogenic viruses. Compared with wild animals, poultry have more frequent interactions and therefore opportunities to transmit their viruses to human. Methods: To study the viromes of different types of poultry in Hainan, China, we used metagenomics for deep viral nucleic acid sequencing of the faecal samples collected from chickens, ducks and pigeons from a live poultry market in Haikou. Result: The poultry viromes were identified by sequence similarity comparisons of viral reads (BLASTxE score, <5) against viral reference database. A total of 15 309 viral reads were obtained, approximately 13 063, 1 370 and 876 viral reads were generated from the chicken, duck, pigeon faecal samples, respectively. The majority of the sequences were homologous to the animal virus of Adenoviridae, Herpeaviridae, Picobirnaviridae, Reoviridae, Retroviridae, Circoviridae, Paramyxoviridae, Astroviridae, Caliciviridae, Coronaviridae, Picornaviridae, and Orthomyxoviridae. The VP4 and VP7 segments of a pigeon rotavirus, similar to fox rotavirus in group A, were sequenced and phylogenetically analyzed. The near full genome of a pigeon circovirus was also analyzed. Conclusion: The major types of poultry in a Haikou harbor many different families of viruses in their feces which may have the potential for interspecies transmissions. Further studies should be conducted to identify the most prevalent and important viruses among a larger number of poultry in Haikou and other areas in Hainan.
Authors and Affiliations
Fei-fei Yin
Keywords
Related Articles
- EP ID EP402101
- DOI 10.4103/1995-7645.243109
- Views 77
- Downloads 0
Antileishmanial activities of caffeic acid phenethyl ester loaded PLGA nanoparticles against Leishmania infantum promastigotes and amastigotes in vitro
Objective: To investigate and compare the antileishmanial effects of CAPE and (CAPE)PLGA NPs on Leishmania infantum (L. infantum) promastigotes and amastigotes in vitro. Methods: Efficacies of CAPE, (CAPE)PLGA NPs and fr...
Asiatic acid-pectin hydrogel matrix patch transdermal delivery system influences parasitaemia suppression and inflammation reduction in P. berghei murine malaria infected Sprague–Dawley rats
Objective: To report the influence of transdermal delivery of asiatic acid (AA) in Plasmodium berghei-infected Sprague Dawley rats on physicochemical changes, %parasitaemia and associated pathophysiology. Methods: A topi...
Comparative genome analysis between Southeast Asian and South American Zika viruses
Objective: To understand the cause for the differences between potentially mild Southeast Asian and the more pathogenic ZIKV in South America. Methods: A comparative genomic analysis was performed to determine putative c...
High expression of TRAF4 in hepatocelullar carcinoma as an independent negative survival and recurrence predictor
Objective: To identify potential tumor markers for the development and recurrence of hepatocelullar carcinoma (HCC), this research studied the relationship between the expression of the tumor necrosis factor receptor-ass...
Antibiotic resistance characteristic and genes analysis of a marine water sourced Klebsiella pneumoniae
Objective: Both antibiotic resistant bacteria (ARBs) and antibiotic resistant genes (ARGs) are considered as one of the most dangerous environmental pollutants in the 21st century by the WHO. This study aims to understan...