A FAST AND SENSITIVE SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF HYDRAZINE IN ATAZANAVIR SULFATE DRUG SUBSTANCE
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 9
Abstract
Objective: To develop a fast and sensitive UV spectrophotometric method for the quantitative estimation of Hydrazine in Atazanavir Sulfate drug substances and validate as per ICH guidelines.Methods: The method was based upon the observation, that a characteristic colour results upon addition of a solution of p-Dimethylaminobenzaldehyde in ethyl alcohol and hydrochloric acid to hydrazine and estimated at absorbance maximum  458 nm in Atazanavir drug substance.Results: The developed method resulted in Hydrazine exhibiting linearity in the range 0.2 to 2.7 µg/g. The Intraday and interday precision is exemplified by relative standard deviation of 0.959 % and 0.947%. Percentage Mean recovery was found to be in the range of 97â€101%, during accuracy studies. The limit of detection (LOD) and limit of quantitiation (LOQ) were found to be 0.2 µg/g and 0.6 µg/g respectively.Conclusion: The present work was aimed to develop a visible spectrophotometric method, which is simple, sensitive, accurate and cost effective to evaluate the quality of the bulk and pharmaceutical formulations.Â
Authors and Affiliations
K Venkata Rao, Raja Gorla, B Sreenivasulu, N Sreenivas, Hemant Kumar Sharma, Raghu Babu Korupolu
Keywords
Related Articles
- EP ID EP579545
- DOI -
- Views 77
- Downloads 0
ACTIVITY ANTI-C. TROPICALIS AND EFFECTS OF THE COMBINATION OF (S)-(-)-CITRONELLAL WITH FOUR ANTIFUNGAL APPLIED IN VULVOVAGINAL CANDIDIASIS
Objective: Assess the antifungal potential of the enantiomer (S)-(-)-citronellal [(S)-(-)-CT] isolated and associated to amphotericin B, fluconazole, itraconazole and miconazole against C. tropicalis from vulvovaginal se...
FORMULATION AND COMPARATIVE EVALUATION OF ONDANSETRON HYDROCHLORIDE MOUTH DISSOLVING TABLETS IN INDIA
Objective: The aim of the present study was to prepare the ondansetron hydrochloride Mouth Dissolving Tablets (MDTs) followed by its comparison with ethical and non-ethical (generic) marketed tablets. Methods: Prior to...
DESIGN AND EVALUATION OF BUCCOADHESIVE CONTROLLED RELEASE FORMULATIONS OF PROCHLORPERAZINE MALEATE
Objective: The Purpose of this work was to design mucoadhesive tablets of prochlorperazine maleate to release the drug in buccal cavity for an extended period of time in order to avoid the first-pass metabolism.Methods:...
DEVELOPMENT AND VALIDATION OF A STABILITY-INDICATING ISOCRATIC REVERSE PHASE-LIQUID CHROMATOGRAPHY ASSAY FOR DETERMINATION OF PHENYTOIN IN BULK AND PHARMACEUTICAL FORMULATIONS
Objective: To develop and validate a stability-indicating reversed phase high performance liquid chromatography (RP-HPLC) assay for the determination of phenytoin in bulk and pharmaceutical dosage forms.Methods: A HPLC i...
ANTI-OBESITY EFFECT OF RESVERATROL IN RATS ON HIGH FAT DIET THROUGH REGULATION OF GENE EXPRESSION OF VISCERAL WHITE ADIPOSE TISSUE
Objective: Obesity is a chronic disease associated with serious health complications including inflammation, insulin resistance, metabolic syndrome, cardiovascular complications and several types of cancers. So this stud...