A new method development and validation for the simultaneous estimation of Zolpidium and Melatonin by using RP HPLC in a bulk and pharmaceutical dosage forms
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2017, Vol 5, Issue 4
Abstract
A simple and selective Liquid Chromatography method was described for the determination of Melatonin and Zolpidem tartarate in tablet dosage forms. Chromatographic separation was achieved on a c18 column using mobile phase consisting of a mixture of 50 volumes of Triethylamine and 50 volumes of Acetonitrile with detection of 280nm. Linearity was observed in the range 20-100 µg/ml for Melatonin (r2 =0.999) and 20-100 µg /ml for Zolpidem tartarate (r2 =0.999) for the amount of drugs estimated by the proposed methods was in good agreement with the label claim. The proposed methods were validated. The accuracy of the methods was assessed by recovery studies at three different levels. Recovery experiments indicated the absence of interference from commonly encountered pharmaceutical additives. The method was found to be precise as indicated by the repeatability analysis, showing %RSD less than 2. All statistical data proves validity of the methods and can be used for routine analysis of pharmaceutical dosage form.
Authors and Affiliations
Pedagamalla Kalyani, P. J. V Sagar
Keywords
Related Articles
- EP ID EP33467
- DOI -
- Views 360
- Downloads 0
Formulation and in-vitro evaluation of levofloxacin floating tablets
Gastroretentive floating tablets of Levofloxacin were successfully prepared with hydrophilic polymers like HPMC K4M, Carbopol 934, HPMC5cps, Xanthan gum. From the compatibility studies for drug and excipients it was ob...
Evaluation of anti arthritic activity of aqueous extract of Hibiscus Platinifolius in albino rats
Rheumatoid arthritis is a chronic, inflammatory disorder that may affect many tissues and organs, but principally attacks flexible (synovial) joints. The process produces an inflammatory responses of the capsule around...
Formulation and evaluation of Escitalopram fast dissolving tablets by direct compression method
The present study is an attempt to select the best possible diluent, disintegrant combination to formulate rapidly disintegrating tablets of Escitalopram which disintegrates in matter of seconds in the oral cavity, the...
Evaluation of anticonvulsant activity of stem bark extract of Tephrosia purpurea
The anticonvulsant potential of Ethanolic extract of stem bark extract of Tephrosia purperia was evaluated in albino mice. The ethanolic extract of stem bark extract of Tephrosia purperia (200 mg/kg and 400 mg/kg), was...
Formulation and evaluation of vildagliptin sustained release tablets
The main aim of proposed work was to develop Vildagliptin matrix tablets, sustained release dosage form, for the treatment anti-diabetic drug. Sustained release formulation is the drug delivery system that is designed...