Alterations of NR1 expression and phosphorylation in the medial prefrontal cortex during neuropathic pain accompanied by negative emotions

Journal Title: Journal of Air Force Medical University - Year 2023, Vol 44, Issue 11

Abstract

Objective To study alterations of the NR1 subunit of N-methyl-D-aspartate receptors (NMDARs) in the expression and phosphorylation in medial prefrontal cortex (mPFC) brain region under the condition of neuropathic pain accompanied by negative emotions, and to provide a theoretical basis for exploring the involvement of NR1 in the occurrence and development of chronic pain accompanied by negative emotions. Methods Spared nerve injury modeling (SNI group) or Sham group were performed in C57BL / 6 male mice. Pain behaviors were detected at 7 and 28 d after modeling, and open field test, elevated plus maze test, and tail suspension test were detected at 28 d after modeling. Subsequently, proteins in mPFC brain region of mice were taken for the determination of NR1 expression and phosphorylation by Western blotting and immunofluorescence staining. Results Compared with the Sham group, SNI mice had decreased mechanical and thermal pain thresholds at 7 and 28 d and showed anxiety- and depression-like behaviors at 28 d, as well as significant upregulation of NR1 phosphorylation. Conclusion Under the condition of neuropathic pain accompanied by negative emotions, the phosphorylation of NR1 subunit of NMDARs in mPFC brain region was significantly upregulated, suggesting that NR1 in mPFC brain region may be involved in the occurrence and development of chronic pain accompanied by negative emotions.

Authors and Affiliations

TIAN Zhicheng, CAO Zixuan, LI Juan, WANG Fei, LIU Kexin, XU Lei, LI Haitao, LUO Peng, LUO Ceng,JIANG Xiaofan

Keywords

Related Articles

Evaluation of damage effect of 300 kg TNT on sheep

Objective To evaluate the damage to sheep at different distances after static explosion of 300kg TNT, and to explore the rules of injury, so as to provide the basis for the evaluation of biological damage effect and the...

Investigation of chronic prostatitis-like symptoms and related risk factors among soldiers at high altitude

Objective To investigate the incidence and related risk factors of chronic prostatitis-like symptoms (CPLS) in soldiers at high altitude. Methods A cluster sampling survey was conducted among soldiers at grass-roots lev...

GSDMD-N mediated hippocampal microglial pyroptosis in a rat model of brain injury after cardiopulmonary resuscitation

Objective To explore the activation and pyroptosis of microglia in the hippocampus after brain injury induced by cardiopulmonary resuscitation (CPR) in rats. Methods A rat model of brain injury after CPR was established...

PVP versus PKP for osteoporotic vertebral compression fractures: a meta-analysis of randomized controlled studies

Objective To conduct a meta-analysis of randomized controlled clinical trials of percutaneous vertebroplasty (PVP) versus percutaneous kyphoplasty (PKP) in the treatment of osteoporotic vertebral compression fractures (...

An auxiliary psychometric method based on eye movement characteristics

Objective In view of the high subjectivity in the traditional psychometric process and the consumption of a lot of manpower and material resources by structured interviews, an auxiliary psychometric method based on eye...

Download PDF file
  • EP ID EP724185
  • DOI -
  • Views 54
  • Downloads 0

How To Cite

TIAN Zhicheng, CAO Zixuan, LI Juan, WANG Fei, LIU Kexin, XU Lei, LI Haitao, LUO Peng, LUO Ceng, JIANG Xiaofan (2023). Alterations of NR1 expression and phosphorylation in the medial prefrontal cortex during neuropathic pain accompanied by negative emotions. Journal of Air Force Medical University, 44(11), -. https://www.europub.co.uk/articles/-A-724185