Antioxidant activity of Algerian Nigella sativatotal oil and its unsaponifiable fraction
Journal Title: The Journal of Phytopharmacology - Year 2017, Vol 6, Issue 4
Abstract
Background/Aim: Nigella sativa(NS) oil is usually considered as oxidatively stable, the aim of this study is to evaluate its antioxidant capacity in vitro and that of its unsaponifiable fraction by several mechanisms. Methods: NS seed total oil (TO) was extracted with n-hexane and this oil was further the matrix to extract the unsaponifiable fraction (UF). TO and its UF were investigated for the antioxidant activity using the following spectrophotometric assays:ABTS (2,2’-azino-bis 3-ethylbenzthiazoline-6-sulfonic acid), DPPH (2, 2’- diphenyl-1-picrylhydrazyl)and H2O2 (Hydrogen peroxide) radicals scavenging tests and lipid peroxidation inhibition capacity assay. All tests were realized by spectrophotometric methods. Results: Quantitative determination of phenols and flavonoids in TO and UF showed that UF has the highest phenolic and flavonoid concentrations with 24,73±0,51 µg GAE/g extract and 8,31±0,40 µg QAE/g extract respectively. All antioxidant assays showed that UF has the strongest antioxidant capacity with; IC50 0,455 ±0,01 mg/ml in ABTS scavenging assay, IC50 113,24 ±0,425 mg/ml in DPPH scavenging assay and IC50 of 0,324±0,003 mg/ml in H2O2 scavenging assay, equally, UF has the strongest Lipid peroxidation inhibition capacity With a percentage inhibition of 57.5%. Conclusion: The results obtained in this investigation showed that the UF has an important antioxidant capacity, which make NS an attractive source of new therapeutic components.
Authors and Affiliations
Fatima Zohra Guergouri, Widad Sobhi, Mustapha Benboubetra
Keywords
Related Articles
- EP ID EP49744
- DOI -
- Views 198
- Downloads 0
Antioxidant and anticancer activities of an Aporphine alkaloid isolated from Alphonsea sclerocarpa
Herein, we report on the anticancer and antioxidant activity of an Aporphine alkaloid isolated from medicinal plant Alphonsea sclerocarpa. Conventional column chromatography and preparative HPLC methods were used to is...
Physicochemical evaluation of male and female plants of Guduchi (Tinospora cordifolia (Willd.) Miers)
Guduchi (Tinospora cordifolia (Willd.) Miers.) is a dioecious creeper, commonly known as „Giloe‟, with significant medicinal importance in the traditional systems of medicine. The plant is dioecious, however, this fact...
Evaluation of anticancer property of mango peel and flesh after formalin treatment
Cancer is life threatening disease an d the second leading cause of death. Various phenolic compounds obtained from plant sources such as fruit, bark, leaf and roots acts against cancer but when such fruits are treated...
Therapeutic activity of Thonningia sanguinea aqueous extract, Vahl on an experimental colibacillosis in chicken
Broiler breeding is faced with serious diseases, including colibacillosis, which is the leading cause of antibiotic therapy and the second leading cause of mortality in poultry after salmonellosis. It is caused by avian...
Evaluation of some plant extracts for nemato-toxic potential against juveniles of Meloidogyne incognita in vitro
Aqueous leaf extracts were utilized to assess the nematicidal or nematostatic property on second stage juvenile of Meloidogyne incognita. The juvenile were incubate at various concentration of leaf extract viz., 250, 50...