Attempt to amplify sequence of steroid 21-hydroxylase in several bird species using degenerate primers flanking the CYP21 gene of mammals
Journal Title: Zeszyty Naukowe Uniwersytetu Przyrodniczego we Wrocławiu, Biologia i Hodowla Zwierząt - Year 2011, Vol 583, Issue 0
Abstract
In this paper attempt to amplify and sequencing of the CYP21 gene in several species of birds. Degenerate primers were used, designed based on known sequences of mammals. In the pilot study, in PCR, mammalian DNA was used. Result of the test was positive. Further studies focused on the DNA of birds. PCR reactions were carried out. Selected products were purified and sequenced. Sequences were obtained from pheasant ([i]Phasianus colchicus[/i]), guinea fowl ([i]Numida meleagris[/i]), turkey ([i]Meleagris gallopav[/i][i]o[/i]), pigeon ([i]Columba livia[/i]) and chicken ([i]Gallus gallus[/i]), but they did not show the expected homology with known sequences of CYP21 gene.
Authors and Affiliations
Krzysztof Grabowski, Tomasz Strzała, Barbara Kosowska
Keywords
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