Biochemical Modulations in Duttaphrynus melanostictus Tadpoles, Following Exposure to Commercial Formulations of Cypermethrin: An Overlooked Impact of Extensive Cypermethrin use
Journal Title: Journal of Applied Biology & Biotechnology - Year 2016, Vol 4, Issue 6
Abstract
Extensive application of pesticides in agricultural and domestic zones has contributed to environmental contamination globally. With aquatic bodies being ultimate recipients of pesticide residues, the inhabiting fauna are known to be largely affected due to their proximity and inevitable exposure. The present investigation was aimed to examine the effect of sublethal (1.11 µg/L) concentration of cypermethrin on biochemical cluster of tadpoles of Duttaphrynus melanostictus. Significant changes were observed in total, soluble and structural protein fractions following cypermethrin exposure to subacute (1, 2, 4 and 6 days) durations in whole animal biochemical composition of Duttaphrynus melanostictus. Marked elevation in free amino acid level was observed at all the exposure tenures. Generation of reactive oxygen species with endpoint induction of oxidative stress were evidenced by decrease in activity of catalase, glutathione-S-transferase and increased levels of hydrogen peroxide, reduced glutathione and malodialdehyde levels. The outcome clearly suggests the increased susceptibility of Duttaphrynus melanostictus tadpoles to sublethal concentrations of cypermethrin, thus implicating the toxicant to possess detrimental health effects on Duttaphrynus melanostictus species. The study may contribute in environmental monitoring and assessment of water bodies with possible cypermethrin contamination.
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Comparative analyses of genomic DNA extracted from freshwater fish tissues preserved in formaldehyde and alcohol in different periods of time
High yields of extracted DNA from animal tissues for a number of molecular research work are depended on acquiring tissues that should not highly degraded and must give a sufficient yield of DNA. Current experiments were...
Plant Growth Promoting Activity of Pink Pigmented Facultative Methylotroph - Methylobacterium extorquens MM2 on Lycopersicon esculentum L.
Pink pigmented facultative methylotrophs (PPFMs) are diversified group of microorganisms that promote plant growth by producing indole acetic acid (IAA) and cytokinins. The objective of the present study is to isolate an...
Fermentative Production of Polyhydroxyalkanoates (PHAs) from Glycerol by Zobellella taiwanensis Azu-IN1
Polyhydroxyalkalonates (PHAs) are biodegradable thermoplastics that are receiving immense attention as an alternative to petroleum derived plastics. The present study aimed to produce PHA using cheap and ecofriendly biod...