Comparative analysis of genetic structure of ukrainian paddlefish (polyodon spathula) populations
Journal Title: Біоресурси і природокористування [Biological Resources and Nature Management] - Year 2018, Vol 10, Issue 3
Abstract
The comparative analysis of the genetic structure of three paddlefish populations was carried out by using microsatellite DNA markers. As the result, Kherson population (25 allelic variants) was the most polymorphic, and the least polymorphous was Chernihiv population (21 allelic variants). It was identified that the maximum number of alleles per locus (Na) at 6,25 was for the population from Kherson region, and the minimum Na at 5,25 was for the population from Chernihiv region. According to the analysis of levels of heterozygosity for each of three Ukrainian populations, it was determined the predominance of the observed heterozygosity (Ho = 0,730), over the expected heterozygosity (He = 0.619). The mean values of fixation index (Fis) ranged from Fis = -0,176 (Chernihiv population) to Fis = -0,129 (Vinnytsia population), indicating the high level of the genetic polymorphism in paddlefish artificial populations under aquaculture conditions. The mean values of PIC index indicated that the investigated loci were polymorphic (P>0,550). The mean combined probability of exclusion (CPE) for the paddlefish populations was 0,912, which indicates 91,2% of the information content of the panel of analyzed microsatellite DNA markers.
Authors and Affiliations
Kh. Kurta, O. Malysheva, V. Spyrydonov
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