Cross-sectional study evaluating the patterns of queries received by a newly established drug and poison information center in a tertiary care hospital
Journal Title: National Journal of Physiology, Pharmacy and Pharmacology - Year 2018, Vol 8, Issue 6
Abstract
Background: In recent decades, Pseudomonas aeruginosa has emerged as a multidrug-resistant bacteria by acquiring intrinsic resistance to a number of antimicrobial agents by uses distinctive resistant mechanisms to all the available antibiotics, which include metallo-β-lactamases (MBL) production, extended spectrum β-lactamase production, Amp C production, decreased permeability, altered penicillin binding proteins and rarely, and over expression of efflux pumps. Aims and Objectives: The aim of the study was to know the presence of IMP gene among P. aeruginosa isolates and to determine the carbapenem resistance mechanisms in carbapenem-resistant isolates of P. aeruginosa collected from sputum, blood, urine, pus, in Chennai, India. Materials and Methods: A total of 20 of non-repetitive clinical isolates of P. aeruginosa were collected and processed for biochemical tests and confirmed. Antibiotic susceptibility testing was determined by Kirby Bauer disc diffusion method. P. aeruginosa isolates were detected for the presence of blaIMP gene by polymerase chain reaction analysis. Results: Of the 20 clinical isolates of P. aeruginosa, 9/20 (45%) isolates were from sputum, 5/20 (25%) from blood, 3/20 (15%) from urine, and 3/20 (15%) from pus. Only 2/20 (10%) isolates showed sensitivity to imipenem. Other than that, for all other antibiotics isolates showed complete resistance 20/20 (100%). 17/20 (85%) clinical isolate of P. aeruginosa was found to possess blaIMP gene. Conclusion: The early detection of MBL‑producing P. aeruginosa may help in appropriate antimicrobial therapy and avoid the development and dissemination of these multidrug-resistant strains. However, to derive a conclusion, a more number of isolates are recommended and even other types of genes are also screened.
Authors and Affiliations
Varshan Raj, Gopinath Pa, Ashish R Jain
Keywords
Related Articles
- EP ID EP489611
- DOI 10.5455/njppp.2018.8.010039012018
- Views 76
- Downloads 0
Impact of simulation-based teaching of applied physiology of the cardiovascular system on the undergraduate medical student
Background: The undergraduate medical student, in his I year of studies, is required to know about Applied Physiology. The teaching of Applied Physiology concepts that occur in real life situations in a classroom is unde...
Eugenol efficacy in preventing nicotine-induced seizures in mice
Background: Seizure is the most common neurological disorder after stroke. In fact, the seizure is a chronic neurological disorder affected 1-2% of the global population. Given the side effects and toxicity of synthetic...
Pain sensitivity and cardiovascular reactivity to the experimental induced cold pressor pain during different phases of menstrual cycle in young Indian females
Background: Many previous research studies have shown a higher prevalence of chronic pain conditions as well as more pain sensitivity or lesser pain thresholds (PTh) among women as compared to men. It might be related to...
Cardiovascular changes in medical students: Examination stress
Background: Functioning capability of normal healthy individual can reduce due to stress which can be observed by the changes in their physiological parameters. Medical students do have huge burden of academic stress dur...
Autonomic cardiovascular regulation in Parkinson’s disease by head-up tilt test - A cross-sectional study
Background: Autonomic failure is an integral component of Parkinson’s disease (PD), and orthostatic hypotension (OH) is commonly observed in advanced cases of PD. OH may be the result of underlying cardiovascular autonom...