DEVELOPMENT AND VALIDATION OF UV- SPECTROSCOPIC METHOD FOR AMLODIPINE BESYLATE, OLMESARTAN MEDOXOMIL, HYDROCHLOROTHIAZIDE IN PURE AND TABLET DOSAGE FORM
Journal Title: Int J of Pharm Res & Analy - Year 2014, Vol 4, Issue 5
Abstract
The objective of the present study to develop and validation of Amlodipine Besylate, Olmesartan Medoxomil, Hydrochlorothiazide in bulk and in combined tablet formulation by UV Spectrophotometric method. Blood pressure lowering drugs such as Amlodipine Besylate, Olmesartan Medoxomil and Hydrochlorothiazide dissolved in methanol (1000g/ml). The stock solutions were further diluted with distilled water to get 10 g/ml scanned in the Double beam UV Spectrophotometer. Amlodipine Besylate estimated at 255 nm, 315 nm and 365 nm, Hydrochlorothiazide at 255 nm and 315 nm and Olmesartan Medoxomil at 255 nm were used and the absorbance corrected for interference method was applied. Hence, Hydrochlorothiazide was found at 315 nm and Olmesartan Medoxomil found at 255 nm. Calibration curve was plotted by using concentration versus absorbance. Range of Amlodipine Besylate was 1 – 5 g/ml. The absorbance of these solutions were measured at 365 nm, 315 nm and 255 nm respectively. Hydrochlorothiazide obeys Beer’s law in the range of 2.5 – 12.5 g/ml and measured at 255 nm and 315 nm. Whereas range of Olmesartan Medoxomil was 4 – 20 g/ml and measured at 255 nm. Correlation coefficient value of these drugs found to be 0.99992.The amount of Olmat AMH (Amlodipine Besylate, Hydrochlorothiazide and Olmesartan Medoxomil) tablets was found to be 99.78 ± 1.1367, 100.90 ± 0.7190 and 99.88 ± 0.5308 for Amlodipine Besylate, Hydrochlorothiazide and Olmesartan Medoxomil respectively. The % RSD (Relative Standard Deviation) values were found to be 1.1392, 0.7126 and 0.5315 for Amlodipine Besylate, Hydrochlorothiazide and Olmesartan Medoxomil respectively. The % RSD value of intraday and inter day analysis were found to be 1.7430 and 0.8715 for Amlodipine Besylate, 0.8361 and 0.6869 for Hydrochlorothiazide and 0.7288 and 0.6542 for Olmesartan Medoxomil. The accuracy of the method was performed by recovery studies. The percentage recovery was found to be in the range of 100.23– 101.27% for Amlodipine Besylate, 99.99 – 100.10% for Hydrochlorothiazide and 99.94 – 100.05% for Olmesartan Medoxomil
Authors and Affiliations
Jothieswari D , Sasi Moulika G, , Amulya R , Chandra Sekhar G , Nagarjun P, , Ravi Chandra Kumar A , Chandraobulreddy B , Bhaskar Reddy K
Keywords
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