EFFECT OF 6-BENZYL AMINO PURINE HORMONE ON THE SHOOTING GROWTH OF OCIMUM GRATISSIMUM L.
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2014, Vol 5, Issue 2
Abstract
BAP (benzyl amino purine) is a cytokine hormone. It causes the shooting effect in plants. We used different concentrations of BAP in MS (Murashige’s and Skoog) media, which stimulate shoots regeneration in different explants of Ocimum gratissimum. The nodal explants and shoot tips were taken and sterilized using bavistien, tween 20 and mercuric chloride. Then explants were introduced into MS media containing various concentrations of BAP concentrations. 0.5 mg/l BAP showed the best performance of proliferation by inducing shoots in 95 % cultured nodal explants. They produced the highest number (12.0 ± 0.5) of shoots per culture on the medium with 2.3 ± 0.29 cm average length of shoots per culture. On the same medium, shoot tip explants, produced shoots in 75 % of the culture. They produced the highest number (6.9 ± 0.23) of shoots per culture, their average length being 1.8 ± 0.29 cm. This study supports the rapid multiplication of this useful medicinal plant by in vitro conditions.
Authors and Affiliations
Sheelu Monga , Neeraj Sethi , Sushila Kaura , Milind Parle , Sarita Lohan
Keywords
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- EP ID EP152074
- DOI 10.7897/2230-8407.050222
- Views 140
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