Effect of Ligustrum lucidum extract on lipid deposition in the liver of laying hens in late laying period
Journal Title: Journal of Henan Agricultural University - Year 2022, Vol 56, Issue 1
Abstract
[Objective] This experiment was conducted to investigate the effects of Ligustrum lucidum extract (LLE) on antioxidation, lipid metabolism and liver microstructure of laying hens fed with high-energy and low-protein diet. [Method] A total of 360 Hy-Line Brown laying hens aged 500 days were randomly divided into 6 groups with 3 replicates in each group and 20 chickens in each replicate. The control group (I group) was fed with corn soybean meal basal diet, and the experimental groups Ⅱ, Ⅲ, Ⅳ, Ⅴ and Ⅵ were fed with high energy and low protein diet, and the diets were supplemented with 0, 500, 1 000, 1 500 and 2 000 mg·kg-1 of Ligustrum lucidum extract respectively. The experiment lasted for 30 days. Blood was collected from wing vein, liver and abdominal fat were dissected, and the liver index, abdominal fat percentage, liver fat percentage and serum and liver antioxidant indexes were detemined. And hematoxylin-Eosin(HE) staining and fluoroscopic electron microscopy were used to study the liver fat deposition and subunit lesions. [Result] 1) Compared with the control group, the liver index, abdominal fat rate and liver fat rate of experimental group Ⅱ significantly increased by 54.02%, 170.41% and 181.60%, respectively. 2) Dietary supplementation of 1 500 mg·kg-1 LLE could significantly increase GSH-Px levels in serum and liver, and decrease MDA levels. 3) Ligustrum lucidum extract had no significant effect on serum TG and TC levels; however, dietary supplementation of Ligustrum lucidum extract at 1 000 mg·kg-1 and 2 000 mg·kg-1 levels significantly decreased the levels of TG and TC in liver, and supplementation at 500, 1 000, 1 500 and 2 000 mg·kg-1 levels significantly reduced the level of ALT in serum and the level of AST and ALT activity in liver.4) The diet with metabolizable energy 11.75 MJ·kg-1 and crude protein 13% could cause liver fat accumulation, mitochondrial swelling, matrix reduction and other micro pathological changes in the late laying period of laying hens, while the addition of Ligustrum lucidum extract could prevent and alleviate the above pathological changes. [Conclusion] The results suggested that dietary supplementation of Ligustrum lucidum extract could improve the antioxidant capacity, lipid metabolism and mitochondrial function of fatty liver syndrome in laying hens at the late laying stage.
Authors and Affiliations
Xiaoli LI, Wanling HE, Yibo WANG, Wang LI, Ke DING, Yanbo MA, Yuanxiao LI, Pinghua CAO, Longmei ZHAO
Keywords
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- EP ID EP769021
- DOI 10.16445/j.cnki.1000-2340.20211209.001
- Views 2
- Downloads 0
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