Effect of phenolization on sugarcane explants (Saccharum spp. Hybrid var. Sp 70-1284) on callus formation
Journal Title: Biotecnología Vegetal - Year 2002, Vol 2, Issue 1
Abstract
Sugarcane (Saccharum spp. hybrid) is the crop were more work has been done on biotechnological aspects because of its economical importance among the comercial crops. When the explants from the field of the hibrid SP 70-1284 are used and manipuled, the lesions produced by the incicion become oxidable. With the objetive to reduce the phenolization that is present in the develoment of callogenesis, a protocol was tested, which showed good results when the spindles were submerged and sectioned in a solution with 200 mg.l-1 at citric acid, as well as the adition of the antioxidant at 50 mg.l-1 to the in vitro culture medium. The section of the explants that generated the greated amount of fresh biomass of callus and the best quality of them were the more young leaves situated above the meristen in the first 5 cm.
Authors and Affiliations
Apolonio Valdez Balero, Pedro Orellana, Leonardo García, Novisel Veitia, Idalmis Bermudez-Caraballoso, Lourdes R. García, Yenni Padrón
Keywords
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