In vitro establishment of Eugenia squarrosa: an endemic species of Santa Clara (Cuba) in n extinction danger
Journal Title: Biotecnología Vegetal - Year 2004, Vol 4, Issue 1
Abstract
The tissue culture techniques can be supplemented with others that are carried out in the cultivation in situ and being applied in combined way to give solution to the extinction of different species. Eugenia squarrosa (Ekman & Urban) it is an endemic species of Cuba in extinction danger due to the urbanization of their natural habitat, which have been reduced to a few hectares. The aim of this work was to achieve the establishment in vitro of this species. Seeds and young branches were collected starting from plants in its natural habitat. The effect of three concentrations of NaOCl (2.0, 2.5, 3.0%) during 20 minutes in the disinfection of the seeds was studied. For the disinfection of the buds a treatment with alcohol to 70% was used during two minutes previously to the disinfection with NaOCl. The effect of three concentrations of this NaOCl was studied (1.0, 2.0, 3.0%) during 10 minutes. The present microbiota was characterized in the contaminated branch. The 100% of disinfection of the seeds was achieved in all the treatments studied. The bigger explants percentage free of contaminant (88.6%) was obtained in the treatment with 3% of NaOCl. However the biggest percentage of survival (45.7%) was obtained when 2.0% of NaOCl was used. The establishment in vitro of the species was achieved starting from seeds and buds collected of field plants.
Authors and Affiliations
Elisa Quiala, Grecia Montalvo , Jesús Matos, Reynaldo Mederos , Yelenys Alvarado, Manuel de Feria, Maité Chávez
Keywords
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