Effects of 5-aza-2′-deoxycitydine on proliferation of prostate cancer cell line PC3 and transcriptional regulation of tumor suppressor gene GSTP1 and RASSF1A
Journal Title: Di-er Junyi Daxue Xuebao - Year 2009, Vol 30, Issue 3
Abstract
Objective:To observe the effects of 5-aza-2′-deoxycitydine (5-aza-CdR) on the proliferation and transcription of tumor suppressor gene GSTP1 and RASSF1A in prostate cancer cell line PC3. Methods: The status of 5′CpG island methylation of RASSF1A and GSTP1 genes in PC3 was analyzed by methylation specific PCR (MSP) before treatment with 5-aza-CdR. RASSF1A and GSTP1 mRNA were quantified by real-time PCR during the demethylation process by 5-aza-CdR. MTT assay and flow cytometry were used to examine the proliferative activity of PC3 cells before and after 5-aza-CdR treatment. Results: The 5′CpG island methylation of RASSF1A and GSTP1 genes were detected in human prostate cancer cell line PC3. Compared with control group, RASSF1A and GSTP1 mRNA expression had no significant change 24 h after culture with 5-aza-CdR; their expression was up-regulated 48 h after cultured with 5-aza-CdR, with significant difference found between 5 μmol/L and 10 μmol/L 5-aza-CdR groups. Compared with control group, the expression of RASSF1A and GSTP1 mRNA was significantly increased 72 h after cultured with all concentrations of 5-aza-CdR. MTT assay and cell cycle examination indicated that exposure to 5-aza-CdR for 24 h and 48 h resulted in no obvious growth inhibition and cell cycle change; exposure to 5-aza-CdR for 72 h induced significant growth inhibition (P<0.05) and cell cycle change (P<0.05); and cells were arrested at G0/G1 phase. Conclusion: The 5′CpG island methylation of RASSF1A and GSTP1 genes is probably responsible for RASSF1A and GSTP1 silencing in PC3 cells. 5-aza-CdR can inhibit the proliferation of PC3 cells, disturb the cell cycle, and elevate transcription of GSTP1 and RASSF1A.
Authors and Affiliations
Wen-hao WENG, Jun-hua ZHENG, Jun LENG, Zhi LI, Jing-hua LI
Keywords
Related Articles
- EP ID EP83061
- DOI -
- Views 130
- Downloads 0
Risk factors of hypoxaemia in patients with end-stage liver cirrhosis
Objective:To screen for risk factors for hypoxaemia and heptopulmonary syndrome (HPS) in patients with end-stage liver diseases by analyzing the preoperative incidence of HPS,so as to provide evidence for anaesthesia man...
结缔组织生长因子反义寡核苷酸体外对人增生性瘢痕成纤维细胞凋亡的影响
目的:观察结缔组织生长因子(CTGF)反义寡核苷酸(ASODN)体外对人增生性瘢痕组织中成纤维细胞凋亡的影响并探讨其可能的作用机制。方法：体外培养的人增生性瘢痕组织中成纤维细胞, 分为CTGF-ASODN 脂质体治疗组(ASODN treatment, AT)、空白脂...
TOPSIS analysis in evaluation of medical service quality in a Chinese hospital
趋化因子MIP-3α及其受体CCR6在溃疡性结肠炎中的表达及其意义
目的:研究趋化因子巨噬细胞炎症蛋白-3α(MIP-3α)及其受体CCR6在溃疡性结肠炎(UC)中的表达情况以及与UC病变程度和范围的关系,探讨其在UC发病机制中的作用。方法:用免疫组织化学法检测35例活动期UC及20例正常对照石蜡包埋组织中MIP-3α及CCR6...
肝硬化食管静脉曲张破裂出血危险因素的Meta分析
目的:综合分析肝硬化门脉高压症下食管静脉曲张破裂出血的危险因素,为制定相关预防措施提供临床依据。方法：检索1986~2006年国内外针对食管静脉曲张破裂出血危险因素的临床研究,进行Meta分析,计算每个危险因素的优势比值(OR值)及其95%可信...