Effects of exogenous chitosan on maize root architecture classification and physiological parameters under drought stress
Journal Title: Journal of Henan Agricultural University - Year 2023, Vol 57, Issue 4
Abstract
[Objective] In order to explore the effect of chitosan on maize growth and root architecture classification under drought stress, and to seek an effective way to improve maize resistance to drought stress. [Method] In this experiment, maize variety Zhengdan 985 was used as the test material, and polyethylene glycol (PEG-6000) was used to simulate drought stress hydroponics test. When the maize grew to two leaves, different concentrations (0, 25, 50, 100, 200, 400 mg·L-1) of chitosan were applied. Maize growth-related indexes, chlorophyll content, photosynthetic parameters, malondialdehyde (MDA), ascorbic acid (AsA) and glutathione (GSH) contents were determined. The root architecture was graded according to root diameter, and their change characteristics were compared. The drought resistance was evaluated comprehensively by calculating the average membership function values of physiological indexes of maize seedlings under different treatments. [Result] Compared with drought treatment, when the concentration of chitosan was 100 mg·L-1, the plant height, shoot fresh weight, root dry weight, photosynthetic rate (Pn), stomatal conductance (Gs), intercellular carbon dioxide concentration (Ci), shoot ascorbic acid, root ascorbic acid and shoot glutathione content reached the maximum, which were significantly increased by 14.47%, 85.61%, 66.67%, 90.10%, 38.89%, 106.99%, 49.52%, 25.54% and 18.42%, respectively. The root length (RL), root surface area (SA) and root volume (RV) of the Ⅲ diameter class interval were significantly increased when the concentration of chitosan was 100 mg·L-1. [Conclusion] Under the condition of PEG-6000 simulated drought stress, the application of different concentrations of exogenous chitosan could effectively improve the growth of maize seedlings under drought stress. Among them, the application of 100 mg·L-1 exogenous chitosan reduced the content of malondialdehyde, increased the content of ascorbic acid and glutathione, enhanced the antioxidant system of maize seedlings, improved the content of Pn, Gs, Ci, chlorophyll, dry and fresh weight of plants, increased the RL, SA and RV of grade Ⅲ diameter range, and strengthened the tolerance of maize seedlings to drought stress.
Authors and Affiliations
Haitao LIU, Qingsong YUAN, Chang WEI, Qiujuan JIAO, Zhengyang XU, Jingjing ZHANG, Gezi LI, Xuehai ZHANG, Beibei ZHENG, Ying JIANG
Keywords
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- EP ID EP769236
- DOI 10.16445/j.cnki.1000-2340.20230411.001
- Views 2
- Downloads 0
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