Efficacy of anti-microbial agents on vaginal microorganisms and reproductive performance of synchronized estrus ewes
Journal Title: Asian Pacific Journal of Reproduction - Year 2017, Vol 6, Issue 3
Abstract
Objective: To isolate and identify microflora and fungal species at different phases during estrus synchronization of ewes and estimate their prevalence; compare the effectiveness of antimicrobial administration to intravaginal sponge on the changes in the vaginal microorganisms and reproductive performance. Methods: Sixty Egyptian ewes were allocated into three equal groups (G: 1, 2 and 3). G1 was inserted with vaginal sponge containing medroxyprogesterone acetate and served as control; without antimicrobial additive. The other two groups were treated as G1, but sponges were previously injected with ciprofloxacin (G2), while sponges of G3 were injected with ciprofloxacin and clotrimazole. Vaginal swabs were collected from each treated ewe, prior sponge insertion, at sponge withdrawal and 48 h later for microbiological investigation and bacterial count. On the day of sponge removal, 300 IU/eCG was administered for each treated ewe. The identified bacterial strains before sponge insertion were tested for sensitivity with antimicrobial disks. Results: Bacterial isolates before sponge insertion were more sensitive to ciprofloxacin. Frequencies of ewes in estrus; the interval from sponge withdrawal to onset estrus and the duration of estrus were statistically similar among treated groups. The pregnancy rate in G2 (100.0%) was higher than G1 (66.7%) and G3 (82.4%). The total bacterial count before sponge insertion was similar between all treatments and increased significantly in all groups on the day of sponge withdraw. The prevailing bacteria on D0, D14 and 48 h after sponge removal for all treated groups were Staphylococcus spp. followed by Escherichia coli. Regarding to fungus species, percentages of isolation increased from 5.00% (before sponge insertion) to 100.00% and 88.89% at sponge withdraw for G1 and G2, respectively. In G3, the fungus was declined from 10% (before sponge insertion) to 5% (at sponge removal). Conclusions: The concomitant treatments by antimicrobial to the vaginal sponge which used for estrus synchronization in ewes can improve reproductive performance. Asian Pacific Journal of Reproduction 2017; 6(3): 121-127 Asian Pacific Journal of Reproduction Journal homepage: www.apjr.net How to
Authors and Affiliations
Mohammed KM, Nabih AM, Darwish GM
Keywords
Related Articles
- EP ID EP240506
- DOI 10.12980/apjr.6.20170305
- Views 207
- Downloads 0
Effect of hematopoietic stem cell on tumor necrosis factor-毩expression, spiral artery remodeling and placental apoptosis in lead-exposed pregnant mice
Objective: To evaluate the effectiveness of hematopoietic stem cell (HSC) on TNF-毩 expression, spiral artery remodeling, and placental apoptosis. Methods: The study used pregnant mice divided to five groups of treatment...
Comparison of serum reproductive hormones, antioxidants, PGE2 and PGF2-α between primary and secondary infertile women in Calabar
Objective: To compare the prolactin, progesterone, luteinizing hormone, follicle stimulating hormone, estradiol, antisperm antibodies, tumor necrosis factor α, total antioxidant capacity, prostaglandin E2 and F2α between...
Antifertility properties of Centella asiatica ethanolic extract as a contraceptive agent: Preliminary study of sperm proteomic
Objective: To investigate the antifertility properties of Centella asiatica L. ethanolic extract and identify the sperm proteomic changes in treated male rats. Methods: A total of 32 male Sprague-Dawley rats were divided...
Non-ischemic priapism in dog: Case report
Bulldog with unilateral cryptorchidism was admitted to our clinic suffering from a persistent erection for 2 d. The animal was left for the first time for breeding without observation. Penis was hyperaemic, oedematous wi...
Live birth rates of assisted reproductive technology treatment and spontaneous conception among subfertile couples in Singapore: A follow-up study
Objective: To explore the potential predictors of a live birth (LB) outcome among subfertile couples of Asian ethnicity undergoing the first fertility treatment cycle; to assess the cumulative live birth rates after succ...