Evaluation of anti-inflammatory activity of ethanolic extract of vitex negundo flowers in experimental animals
Journal Title: Indian Journal of Pharmacy and Pharmacology - Year 2017, Vol 4, Issue 4
Abstract
Introduction: The objective of the present study was to evaluate the anti-inflammatory activity of ethanolic extract of vitex negundo flowers (EEVNF) in experimental animals. Methods: Acute toxicity test of extract was done following Organization for Economic co-operation and development guidelines (OECD guidelines). Wistar albino rats of either sex weighing 150-250 grams were used for the study. Animals were randomly grouped into 5 groups consisting of 6 animals each. The in vivo acute anti-inflammatory activity was studied using the Carrageenan induced paw edema method. Normal saline was administered to the first group, diclofenac sodium (25mg/kg body weight{b.w} of rat ) was administered to the second group and doses of 100mg/kg, 200mg/kg and 400mg/kg b.w of rat of EEVNF were administered to the third, fourth and fifth groups respectively. All the drugs were given orally. 0.05ml of 1 % w/v carrageenan was used to induce paw edema. After l hr interval all the animals were given 0.05ml of 1 % w/v carrageenan in normal saline solution into the subplantar aspect of the left hind paw. Right hind paw served as the control for the same animal. The paw volume was measured by using digital plethysmometer by measuring fluid displacement at 1hr, 3hrs & 5hrs after Carrageenan injection. Results: Test drug produced reduction in paw volume which was significant (p<0.05) at 3rd and 5th hr respectively at doses 100mg/kg, 200mg/kg and 400mg/kg b.w of rat when compared to diclofenac sodium. Conclusion: EEVNF has signiï¬cant anti-inflammatory activity.
Authors and Affiliations
Yasmeen A Maniyar, Dasari Sriraj
Keywords
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- EP ID EP295774
- DOI 10.18231/2393-9087.2017.0037
- Views 107
- Downloads 0
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