EVALUATION OF ANTIBACTERIAL, ANTIFUNGAL, ENZYME INHIBITION AND HEMOLYTIC ACTIVITIES OF CARYOPTERIS ODORATA FRACTIONS
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2013, Vol 4, Issue 12
Abstract
The purpose of this study was to explore the antibacterial, antifungal, enzyme inhibition and hemolytic activities of various fractions of Caryopteris odorata (Ham. Ex Roxb.). The methanolic extract of plant was dissolved in distilled water and partitioned with n-hexane, chloroform, ethyl acetate and n-butanol sequentially. Antibacterial activity was checked against Staphylococcus aureus, Bacillus subtilis, Pasturella multocida and Escherichia coli by the disc diffusion method using streptomycin sulphate, a standard antibiotic, as positive control. Polar fractions showed good antibacterial potential against these strains. These fractions also showed good MIC values. Antifungal activity was studied against four fungi i.e. Aspergillus niger, Aspergillus flavus, Ganoderma lucidum and Alternaria alternata by the disc diffusion method using fluconazole, a standard antifungal drug, as positive control. Ethyl acetate fraction was found active against A. niger and G. lucidum while chloroform and n-butanol fractions were active against A. flavus. Enzyme inhibition studies were performed against four enzymes i.e. α-glucosidase, butyrylcholinesterase, acetylcholinesterase and lipoxygenase. Ethyl acetate and n-butanol soluble fraction exhibited good activity against α-glucosidase with IC50 values 79.77 ± 0.97 and 82.77 ± 0.88 respectively. Chloroform soluble fraction showed moderate activity against butyrylcholinesterase (IC50 69.71 ± 0.95) while chloroform and ethyl acetate soluble fraction exhibited good activity against the lipoxygenase with IC50 values 65.28 ± 0.57 and 78.71 ± 0.69 respectively. Ethyl acetate fraction showed high toxicity i.e. 85.14 ± 0.83 %.
Authors and Affiliations
Muhammad Abbasi, Aziz-ur Rehman, Viqar Ahmad, Tauheeda Riaz, Farah Khlaid
Keywords
Related Articles
- EP ID EP131238
- DOI 10.7897/2230-8407.041203
- Views 127
- Downloads 0
ULTRADEFORMABLE LIPID VESICLE AS A NOVEL DRUG DELIVERY SYSTEM
Various new technologies have been developed for the transdermal delivery of some important drugs. Transdermal route will always remain a lucrative area for drug delivery.The transdermal route of drug delivery has gained...
QUALITATIVE PHYTOCHEMICAL SCREENING AND ANTIFUNGAL ACTIVITY OF CARICA PAPAYA LEAF EXTRACT AGAINST HUMAN AND PLANT PATHOGENIC FUNGI
Plants have been explored extensively all over the globe in quest of a novel bioactive compound that could a good therapeutic candidate treating infectious diseases especially against drug resistant microbes. Qualitative...
PRELIMINARY PHYTOCHEMICAL ANALYSIS AND STUDY ON ANTIOXIDANT PROPERTY OF AMARANTHUS CAUDATUS LINN.
The present study was carried out for the analysis of dietary antioxidants viz phenolics and flavonoids from the leaves of A.caudatus which is an annual herb and a popular leafy vegetable. The antioxidantpotency of the s...
EVALUATION OF OXIDATIVE STRESS MARKERS IN CHRONIC KIDNEY FAILURES OF SOUTH INDIAN POPULATION
Oxidative stress defines an imbalance between the formation of reactive oxygen species and antioxidants. The existence of oxidative stress and higher incidence of cardiovascular diseases (CVD) in association with uraemia...
PHARMACOLOGICAL ACTIVITIES OF AERVA LANATA: A PERSPECTIVE REVIEW
India has the richest bio-resource of medicinal plants that have long life history of application in the treatment of diseases from the ancient times. The drugs that are obtained from the medicinal plants have been well...