Evaluation of antifungal, hemolytic and cytotoxic potentiality of ethyl acetate crude extracts of a novel marine Streptomyces spp. AIAH-10
Journal Title: Scientific Journal of Microbiology - Year 2015, Vol 4, Issue 5
Abstract
To face newly generated diseases, searching for new, safe and effective bioactive molecules is urgent and marine microbial flora may be a potential source. The present study was designed to isolate marine microorganisms (AIAH-1 to AIAH-29) from the soil of mangrove forest Sundarbans, Bangladesh by serial dilution method using isolation media. Among them, AIAH-10 was selected for further study due to its promising antibacterial activity (done by streak plate and plug technique method) against a series of pathogenic bacteria. On the basis of morphological, cultural and biochemical analysis, the strain AIAH-10 belongs to Streptomyces spp. Secondary metabolites of the strain was obtained by small scale fermentation process. Antifungal activity of the extracts was performed by disc diffusion method. The crude ethyl acetate extract (50 mg/disc) showed significant antifungal activity against Aspergillus niger, Candida albicans and Saccharromyces cerevaceae (14, 12, 10 mm zone of inhibition respectively). No hemolytic activity was found of the extracts towards the human erythrocytes. During cytotoxic study against brine shrimp nauplii (Artemia salina), a dose dependent mortality rate was observed. A 100% larva mortality rate was recorded in 40 mg/ml and more where as LC50 was found to be 6.61 mg/ml.
Authors and Affiliations
Md. Anwarul Haque| Department of Pharmacy, Pabna University of Science and Technology, Pabna-6600, Bangladesh., Ashish Kumar Sarker| Department of Pharmacy, Pabna University of Science and Technology, Pabna-6600, Bangladesh., Mohammad Sayful Islam| Department of Pharmacy, Primeasia University, Banani-1213, Dhaka, Bangladesh., Kartik Ranjon Roy| Department of Pharmacy, University of Rajshahi, Rajshahi-6205, Bangladesh., Netish Kumar Kundu| Department of Pharmacy, University of Rajshahi, Rajshahi-6205, Bangladesh., Abu Syed Md Anisuzzaman| Department of Pharmacy, University of Rajshahi, Rajshahi-6205, Bangladesh.Department of Hematology & Medical Oncology, Winship Cancer Institute, Emory, University, Atlanta, GA 30322, USA., Md. Anwar Ul Islam*| Department of Pharmacy, University of Rajshahi, Rajshahi-6205, Bangladesh.
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- EP ID EP1202
- DOI 10.14196/sjm.v4i5.1843
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