Evaluation of middlebrook 7h11 associated with human or sheep blood for the detection of mycobacterium tuberculosis in sputum samples.
Journal Title: Revista Peruana de Medicina Experimental y Salud Publica - Year 2009, Vol 26, Issue 3
Abstract
[b]Objective.[/b] To evaluate the diagnostic yield of the media Middlebrook 7H11 combined with human or ovine blood in comparison with the Ogawa solid media for the diagnosis of pulmonary tuberculosis. [b]Material and methods.[/b] We evaluated sputum samples of patients with clinical suspicion of pulmonary tuberculosis. The samples were seeded in Middlebrook 7H11 agar associated with human or ovine blood and in Ogawa media. [b]Results.[/b] A total of 130 samples were collected. The positivity for [i]M.tuberculosis[/i] in Middlebrook 7H11/human blood, Middlebrook 7H11/sheep blood and Ogawa was 45.38%, 46.15% and 43.84% respectively. The mean times for growth for the M. tuberculosis were 12.81± 6.52, 13.07 ± 6.63 and 20.14 ± 6.75 days (p< 0,01 for the comparison of agar based medium versus Ogawa). [b]Conclusions. [/b]The combination of Middlebrook 7H11 with sheep or human blood has a comparable diagnostic yield but a shorter growth time than the Ogawa solid media.
Authors and Affiliations
Juan Agapito, Luis Cuadros, Sergio Tarrillo
Keywords
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