Evaluation of the effectiveness of bleach on microbial population of lettuce
Journal Title: Scientific Journal of Microbiology - Year 2013, Vol 2, Issue 9
Abstract
The effectiveness of bleach on the microbial population of lettuce was evaluated. Microorganisms on lettuce before and after washing with bleach were searched and counted and a new dose for cleaning to eliminate a high concentration of bacteria on the lettuce has been proposed to minimize the health risk linked to its consumption. The lettuce samples were collected from farms municipalities V and VI of the District of Bamako. Ten lettuces were collected from different plots and mixed to form a composite sample. The concentration of microbial population was determined by finding and counting the total microflora in 100g of lettuce composite sample before and after washing with bleach different chlorine concentrations for different contact times. Lettuces grown in Bamako are heavily contaminated with microbial population average between 25 to 1363UFC / g of lettuce. The application of the recommended dose of chlorine (133.10-5mg / l) in Mali for the disinfection of fruits and vegetables for 15, 30 and 60 min resulted in a drawdown respectively 94%, 97% and 99% of the initial microbial population lettuce. The recommended dose and double dose of this leading to high rates of drawdown of 97% and 99% of the initial microbial population of lettuce. 1% of the concentration of microbial population remains on the treated leaves. Dose which totally eliminates the microflora is 2.6 mg / l of chlorine applied for fifteen minutes. The new dose we can offer is the application of 2.6 mg / l of active chlorine.Keywords: Lettuce, bleach, efficiency, disinfection, concentration of microbial population.
Authors and Affiliations
S. Traoré*| Research Laboratory of Microbiology and Microbial Biotechnology, Faculty of Science and Technology, University of Science, Technique and Technology Bamako, BPE. Bamako-Mali 3206., F. Samaké| Research Laboratory of Microbiology and Microbial Biotechnology, Faculty of Science and Technology, University of Science, Technique and Technology Bamako, BPE. Bamako-Mali 3206., A. H. Babana| Research Laboratory of Microbiology and Microbial Biotechnology, Faculty of Science and Technology, University of Science, Technique and Technology Bamako, BPE. Bamako-Mali 3206., D. Traoré| Research Laboratory of Microbiology and Microbial Biotechnology, Faculty of Science and Technology, University of Science, Technique and Technology Bamako, BPE. Bamako-Mali 3206., Y. Sanogo| Laboratory of Applied Molecular Biology, Faculty of Science and Technology, University of Science, Technique and Technology Bamako, BPE. Bamako-Mali 3206., K. Maiga| Research Laboratory of Microbiology and Microbial Biotechnology, Faculty of Science and Technology, University of Science, Technique and Technology Bamako, BPE. Bamako-Mali 3206.
Keywords
Related Articles
- EP ID EP1171
- DOI 10.14196/sjm.v2i9.950
- Views 453
- Downloads 24
Evaluation of antifungal, hemolytic and cytotoxic potentiality of ethyl acetate crude extracts of a novel marine Streptomyces spp. AIAH-10
To face newly generated diseases, searching for new, safe and effective bioactive molecules is urgent and marine microbial flora may be a potential source. The present study was designed to isolate marine microorganisms...
Identification of lactic acid bacteria isolated from Tarkhineh, a traditional Iranian fermented food
Tarkhineh is a traditional Iranian fermented product produced from a mixture of doogh and wheat grout. The purposes of the present study were identifying of lactic acid bacteria (LAB) isolated and Changes of lactic acid...
Comparison of capillary and venous blood using blood film microscopy in the detection of malaria parasites: A hospital based study
Malaria mainlydue to Plasmodium falciparum isassociated with great morbidity and mortality in Sub-Saharan Africa especiallyin children under 5 years. In order to eradicate the disease and avoidcomplications that may aris...
Molecular detection of inv A and spv virulence genes in Salmonella typhimurium isolated from human and animals in Iran
In the present study, multiplex Polymerase Chain Reaction (m-PCR), simple PCR assay was used to confirm Salmonella typhimurium and detect Salmonella plasmid virulence (spvA, spvB, spvC) genes in human, bovine and poultry...
Distribution of Aspergillus species among apparently healthy birds in poultry farms in Kaduna state, Nigeria
The study wasconducted to determine the prevalence and distribution of Aspergilllus species among apparently healthy birds in poultryfarms. Trachea swabs from 1500 birds in 52 commercial (10% of birds in eachpoultry farm...