Evaluation of three protocols for hormonal induction of spermiation in yaque Leiarius marmoratus
Journal Title: Revista MVZ Cordoba - Year 2010, Vol 15, Issue 2
Abstract
Objective. To assess the effects of hormonal induction using pituitary carp extract (PCE) and sGnRHa + domperidone (OVA) on the release of seminal fluid and its characteristics. Materials and methods. Treatments were: T1: PCE administered in three doses : 0.2 mg/ kg (0 h), 0.5 mg/kg (9 h) and 2 mg/kg (18 h), T2: 8 mg/kg of PCE (0 h), 5 mg of PCE (24 h) and 0.5 ml of OVA (48 h), T3: single dose of 0.25 ml of OVA and Control: 3 ml of saline solution administered in three doses (0, 24 and 48h). Volume, mass and individual motility, duration of movement, speed and sperm concentration were evaluated using semi-quantitative assessment and Computer Assisted Sperm Analisis (CASA). Results. T1, T2 and T3 showed a significantly greater volume (2.7 ± 0.7, 2.6 ± 0.5 and 1.8 ± 0.4 ml) compared with the control (0.2 ± 0.1 ml). T2 and T3 showed the best results in motility and duration of movement, (95 ± 0.0% for 64.6 ± 3 sec and 89.4% for 63.6 sec respectively). T3 had the highest individual total motility (88.9 ± 4.5%) in contrast to T1 (60.6 ± 5.6%), and equal to T2 and the control (68.2 ± 11.5% and 81.2 ± 1.6%). The percentage of immotile spermatozoa was higher in T1 and T2 than T3 and the control. T3 showed the greatest curvilinear velocity and average velocity of displacement (64.3 ± 9.2 and 57.0 ± 9.6 μm/sec). The sperm concentration was higher (p <0.05) in T3 and the control (6255 ± 1322 and 9.460sptz x103/ìl). Conclusions. Hormonal induction with PCE and OVA, applied individually or combined, are effective for spermiation induction in L. marmoratus.
Authors and Affiliations
Tatiana Mira L, Zoot *| Universidad de los Llanos, Instituto de Acuicultura de los Llanos. Grupo de Investigación sobre Reproducción y Toxicología de los Organismos Acuáticos - GRITOX, km 12 vía Puerto López, Villavicencio, Colombia.Correspondencia: t.mirah@gmail.com, Mauricio Medina R, M. Sc| Universidad de los Llanos, Instituto de Acuicultura de los Llanos. Grupo de Investigación sobre Reproducción y Toxicología de los Organismos Acuáticos - GRITOX, km 12 vía Puerto López, Villavicencio, Colombia., Pablo Cruz C, Ph. D| Universidad de los Llanos, Instituto de Acuicultura de los Llanos. Grupo de Investigación sobre Reproducción y Toxicología de los Organismos Acuáticos - GRITOX, km 12 vía Puerto López, Villavicencio, Colombia.
Keywords
Related Articles
- EP ID EP3158
- DOI -
- Views 408
- Downloads 18
HISTOPATOLOGICAL AND IMMUNOHISTOCHEMICAL STUDY ON NEOSPOROSIS IN BOVINE FETUSES COMING FROM SLAUGHTERHOUSE
Objective. To determine the presence of lesions, antigens or evolved forms compatible with Neospora, through histopathological and immunohistochemical studies in the central nervous system of bovine fetuses from a slaugh...
Goat milk fatty acid composition in the Peninsula of Baja California, Mexico
Objective. The objective of the study was to determine the composition of fatty acids in milk of Creole goats in an extensive production system in an arid region of the peninsula of Baja California and compare it to two...
Single dose of secnidazole treatment against naturally occuring Giardia duodenalis infection in Sakiz lambs
Objective. The purposes of this study were to determine whether secnidazole administered at a single dose of 10 mg/kg, orally, lessens or eliminates Giardia cyst shedding, and to validate the benefit of secnidazole treat...
Fatal parasitosis in blackbucks (Antilope cervicapra): a possible factor risk in hunting units
In February 2012, a reproductive group of 60 adult blackbucks (Antilope cervicapra) from Veracruz, Mexico was relocated to hunting units in eastern and northeastern Mexico. Seven individuals died due to hemorrhagic paras...
RESTRICCIÓN DEL AMAMANTAMIENTO EN VACAS DEL SISTEMA CRÍA LIBRE
In order to increase the pregnancy rate which it was found diminished by the incidence of a long anoestrus postpartum in lactating cows in the farm Andalucía, the technology or «restriction of the suckling period to 30 m...