EXPRESSION PROFILING OF SELECTED MICRO RNAs COUPLED WITH MOLECULAR AND BIOCHEMICAL ANALYSES OF ASIATICOSIDES IN CENTELLA ASIATICA (L.)URB IN VITRO
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2019, Vol 11, Issue 5
Abstract
Objective: Centella asiatica (L.) Urb from Umbelliferae is a potential source of secondary metabolites having immense medicinal value. Asiaticoside is the major therapeutic compound. In the present study, Identification of a possible relationship between concentration/transcript level expression of asiaticoside and concentrations of growth hormones at different growth stages was observed. The current study includes molecular and biochemical evaluation of stress generated in C. asiatica at different time intervals in vitro. Methods: The enhancement in auxin, cytokinin and final asiaticoside content were determined using immunoassay kits for auxin, cytokinin and HPLC analysis respectively. Transcript level expression at different growth phases was carried out using real-time RT-PCR. For isolation of stress-related miRNAs, reverse transcription of total RNA using miScript II RT Kit PCR System was carried out as per instructions. The differential expression of five selected miRNAs was done by Real-Time RT-PCR. The analysis of stress in vitro was done by quantification of Hydrogen Peroxide (H2O2), total phenolics and total antioxidants by H2O2 assay kit, total antioxidant assay kit and Folin Ciocalteau reagent respectively. The final asiaticoside content was determined by HPLC. Results: Differential expression of key genes involved in asiaticoside pathway showed significantly higher transcript expression, which is in correlation with the final asiaticoside content. The enhanced expression of miRNAs and the analysis of H2O2, total antioxidant capacity and total phenolics are suggestive of generation of oxidative stress under controlled conditions. Conclusion: The present study shows a direct correlation between oxidative stress and transcript/phytochemical estimation of asiaticoside content under in vitro conditions.
Authors and Affiliations
JISHA S. , HEMANTHAKUMAR A. S. , GOURI P. R. , SABU K. K.
Keywords
Related Articles
- EP ID EP565232
- DOI 10.22159/ijpps.2019v11i5.32097
- Views 159
- Downloads 0
ROLE OF NITRIC OXIDE (NO) IN CAPSAICIN MEDIATED ANTI-PLATELET ACTIVITY IN IN VITRO, IN VIVO, EX-VIVO MODEL OF PLATELET AGGREGATION ASSAY AND ARTERIAL THROMBOSIS IN RAT: POTENTIAL THERAPEUTIC TARGET?
Objective: Although recent advances in the treatment of congestive heart disease, mortality among patients’ remains a questionable remark. Therefore, we evaluated the role of capsaicin on in vitro and ex vivo platelet...
ASSESS THE PREVALENCE OF RESPIRATORY PROBLEM AND KNOWLEDGE ON HEALTH EFFECTS OF PEOPLE RESIDING NEAR QUARRY
Objective: In the quarry area, there are various suspended particulate matters are mixed with the ambient air in and around. People residing near to that quarry areas are exposed to hazards through the inhalation of airb...
STRUCTURAL EXPLORATION AND PHARMACOPHORIC INVESTIGATION OF PYRAZOLE BASED ANALOGS AS NOVEL HISTONE DEACETYLASE 1 INHIBITOR USING COMBINATORIAL STUDIES
Objective: Histone deacetylase inhibitors (HDACi) have four essential pharmacophores as cap group, connecting unit, a linker moiety and zinc binding group for their anticancer and histone deacetylase (HDAC) inhibition ac...
DEVELOPMENT OF HYDROPHILIC MATRIX TABLET OF CARBAMAZEPINE USING 33 FULL FACTORIAL EXPERIMENTAL DESIGNS
Objective: The study aims at developing the hydrophilic matrix tablet of carbamazepine (CBZ) using an optimization technique. The purpose of the study was to develop the hydrophilic matrix dosage form using the combinati...
ANTIFUNGAL EFFECT OF HYPTIS SUAVEOLENS OIL MICROEMULSION BASED CARBOXYMETHYL MUNGBEAN GEL FOR TOPICAL DELIVERY
Objective: Conventional topical antifungal formulations limit the effectiveness of antifungal therapy. The aim of this study was to formulate effective antifungal microemulsion of H. suaveolens oil based carboxymethyl mu...