First Study of scpB Gene of Streptococcus agalactiae in Misiones, Argentina
Journal Title: Microbiology Research Journal International - Year 2015, Vol 8, Issue 3
Abstract
Aims: Streptococcus agalactiae (GBS) is the leading cause of neonatal infections. GBS presents different surface proteins of antigenic characteristics. These proteins are encoded by several genes associated with virulence and host interaction acting on bacteria involved in invasiveness. The study of these surface protein antigens is important for the understanding of the pathogenesis and epidemiology of the infection. Several of these antigens have been proposed as components of multivalent conjugate vaccines. This study focuses on the presence of scpB gene encoding the C5a peptidase in GBS. Recent studies indicate that C5a peptidase vaccine formulations cause a long-term immune response and prevent GBS infection. Methodology: A total of 200 Streptococcus agalactiae isolates were collected from vaginal or rectal swabs of pregnant women with 35 – 37 weeks gestational age. Conventional biochemical tests and commercial kits for latex particle agglutination (Phadebact Strep B Test-ETC-Bactus International AB Sweden) were used to identify those isolates. GBS strains were preserved in 20% skim milk at -80°C and were recovered to search the gene scpB. The scpB gene was investigated by conventional PCR. PCR products were visualized on 2% agarose gel gel stained with GelRed® (Biotium, Inc.; US) and visualized of bands in UV trans illuminator (MUV Model 21-312-220), using MP (D0017 marker DNA fragments made up of 10 double-stranded DNA molecular size of 100 to 1000 pb-INBIO, Argentina). Results: The presence of the scpB gene was detected in 100% of Streptococcus agalactiae strains studied. Conclusion: Our findings highlight the importance of including C5a peptidase in the design of a regional vaccine. The high specificity of the scpB gene in GBS, and the lack of homologous sequences in other bacterial genera and species, makes it a molecular marker that can be used in the diagnosis of colonization or infection by this microorganism.
Authors and Affiliations
M. E. Laczeski, M. G. Novosak, M. I. Vergara
Keywords
Related Articles
- EP ID EP354493
- DOI 10.9734/BMRJ/2015/17956
- Views 96
- Downloads 0
Protective Effect of Hamamelitannin against Biofilm Production by Methicillin-resistant Staphylococci Isolated from Blood of Patients at Intensive Care Units
S. aureus and S. epidermidis are common pathogens in biofilm related infections of indwelling medical devices. Aim: The aim of this study was to assess the efficacy of vancomycin and clindamycin alone and in-combinatio...
Staphylococcus aureus: Nasal-carriage in Health Care Workers and In-patients with Special Reference to MRSA
Aims: To find out the nasal carriage of Staphylococcus aureus in health care workers and in-patients in a tertiary care center. Study Design: Cross sectional study. Place and Duration of Study: Department of Microbiolo...
Strain Development of Aspergillus brasiliensis Using Physical and Chemicals Mutagenesis for Possible Overproduction of Xylanase, Amylase, Protease and Cellulase under Submerged Fermentation (SmF)
Aims: Xylanase is commonly involved in the complete hydrolysis of xylan which consisted of hemicelluloses and celluloses as important components of plant cell wall. This enzyme is essential in wide range of applications...
The Fallacy of Identification by Neutralization in the Light of Non-Cytopathic Effect Producing Enterovirus Strains
We describe the characterization of an enterovirus isolate recovered from untreated raw sewage in Ibadan, southwest Nigeria in 2010. The isolate was neutralized by specific antisera and consequently identified as Echovir...
Effects of Water Activity on the Radial Growth of Aspergillus niger on Solid Medium
The effects of water activity (aw) on the radial growth of Aspergillus niger on Malt Extract Agar was investigated using sodium chloride and glycerol mixtures. Varied concentrations of each solute was used to modify the...