Functional analysis of miR161.1 by STTM technology in Arabidopsis
Journal Title: Journal of Henan Agricultural University - Year 2022, Vol 56, Issue 6
Abstract
[Objective] The miR161.1 knockdown mutant was obtained by STTM technology. The STTM161.1 mutant plants displayed significant phenotypic alterations. It lays a foundation for the functional study of miR161, and can be applied to sterile seed production of crop hybrid. [Methods] In Arabidopsis, the transgenic lines, STTM 161.1, were generated by the transformation of the constructed pFGC5941-STTM161.1 binary vector. STTM161.1 mutant plants were obtained by genotype and phenotype analysis. Transcriptome analysis was performed to identify the alterations of genome-wide gene expression. [Results] RT-qPCR analysis indicated that miR161.1 down-regulated in STTM161.1 plants, while target genes were significantly up-regulated. Compared with the wild type, STTM 161.1 plants displayed various of phenotypic alterations, including early reproductive age, enlarged leaves, partial male sterility, and seed abortion in fruit pods. The transcriptome analysis revealed that several plastid expression genes were significantly up-regulated, and miR161.1 was mainly involved in multi-ple metabolic pathways, including secondary metabolite biosynthesis, phenylpropane biosynthesis, purine metabolism, and beta-linolenic acid. The differentially expressed genes screened between STTM161.1 and the wild type include a MADS TF encoding gene associated with floral development, and a bHLH transcription factor encoding gene associated with plant floral and pollen development. [Conclusion] miR161.1 was proved to have taken part in the regulation of male sterility, developmental phase transition and seed development in Arabidopsis. 17 up-regulated and 6 down-regulated differentially expressed genes were verified to be possibly related to the functions of miR161.1.
Authors and Affiliations
Weiya LI, Feiyan QI, Juan MENG, Na LI, Xiaoqi LI, Qiusheng WANG, Guiliang TANG, Zhanhui ZHANG
Keywords
Related Articles
- EP ID EP769137
- DOI 10.16445/j.cnki.1000-2340.20221103.002
- Views 15
- Downloads 0
Research progress on main active components and multifunctional utilization of aromatic plants
In this paper, the main active components of common aromatic plants were analyzed, the aroma characteristics of terpenoids, aromatic family of benzene ring structure and fatty acids in aromatic components, the medicinal...
Effects of exogenous chitosan on maize root architecture classification and physiological parameters under drought stress
[Objective] In order to explore the effect of chitosan on maize growth and root architecture classification under drought stress, and to seek an effective way to improve maize resistance to drought stress. [Method] In th...
Study on estimation model of the genetic parameters for body measurements traits in Yu’nong black pigs
[Objective] In order to improve the accuracy of genetic parameter estimation of body measurements traits in Yu’nong black pig and speed up the breeding progress in Yu’nong black pigs. [Method] In this study, the two meth...
Spatio-temporal characteristics of urban expansion in Zhengzhou from 1990 to 2020
[Objective] Analysis of the spatio-temporal characteristics of urban expansion can help achieve precise control of urban expansion and improve the quality of urban development. [Method] The land use data interpreted by m...
Yield effects and phosphorus utilization efficiency of summer maize at different phosphorus application positions
[Objective] To clarify the yield effect and phosphorus (P) utilization efficiency characteristics of summer maize based on the positive point hole P fertilizer application methodology, and thus to provide a theoretical b...