Functional Properties (Acid and Bile Tolerance) and Antibiotic Susceptibility of Lactic Acid Bacteria Isolated from Newborn Calves for the Design of a Probiotic Product
Journal Title: International Journal of Veterinary Science and Research - Year 2015, Vol 1, Issue 1
Abstract
Diarrhea in young calves in dairy farms is one of the main causes of economic losses, morbidity and mortality. The use of probiotic products as feed additives or complements is a novel alternative for the prevention of intestinal syndromes. In order to include beneficial bacteria in the design of a probiotic product, their functional and safety characteristics must be studied. The aim of this work is to evaluate the behavior of the strains in some “in vitro” gastrointestinal conditions such as acid stress and bile salts in the specific physiological concentration of young calves. The antibiotic susceptibility of a group of lactic acid bacteria from calves which were identified due to their beneficial properties was also studied. The strains, genetically identified and used for the resistance assays were: Lactobacillus johnsonii CRL1692, CRL1693, CRL1699, CRL1700, CRL1701 and CRL1706; L. amylovorus CRL1697; L. murinus CRL 1695 and CRL1705; L. mucosae CRL1696 and CRL1698; L. salivarius CRL1694 and CRL1702; and Enterococcus faecium CRL1703. The results of gut resistance assays showed that all the strains were resistant to pH 4 and to a bile salts concentration of less than 0.5%. However, some of them were sensitive to pH 2. The most pH-sensitive strains were found to be L. johnsonii and L. amylovorus, and enterococci. However, pre-treatment at low pH increased the growth rate of the L. salivarius strains. The minimal inhibitory concentration showed that the strains were sensitive to Tetracycline, Erythromycin, Chloramphenicol and Ampicillin, while most of them were resistant to Kanamycin. The results allowed the selection of the most adequate strains to be included in a probiotic product that can be utilized most successfully in young calves.
Authors and Affiliations
Maldonado Natalia C, Nader-Macías María Elena Fátima
Largemouth Bass Pond Culture in China: A Review
Largemouth bass micropterus salmoides natives to freshwater area of North America, which is a kind of famous global economic fish, and belongs to Centrarchidae, Perciformes in taxonomy. The fish has been extensively cult...
Inhibitory Effect of Mentha Longifolia L. Essential Oil against Listeria Monocytogenes Using Transmission Electron Microscopy
Inevitable side effects of chemical food preservatives and drug resistance have increased interests on use of natural preservatives derived from plants. Therefore, in the present paper, the biological properties of Menth...
Mesenchymal stem cells from the domestic ungulates: trends and outliers
Mesenchymal stem cells (MSCs) are a valuable source for regenerative therapy and tissue engineering. MSCs are multipotent adherent stem cells that can be isolated from different adult and fetal tissues. In contrast to hu...
Renal Protective effects of Melatonin in rat treat by Gemcitabine
Nephrotoxic side effects of gemcitabine have been reported in patients receiving this drug medication. Melatonin is a potent free radical scavenger and antioxidant and has protective effects against ischemic damage. In...
Current Status of Grevy Zebra: A Review
Grevy’s zebra is a member of the horse family Equidae and so is closely related to horses, asses and other zebras. They inhabit dry desert regions and open grasslands. Grevy’s zebra is one of the world’s most threatened...