Identification and control of Bacillus sp., Contaminant of in vitro establishment of Guadua angustifolia Kunth
Journal Title: Biotecnología Vegetal - Year 2007, Vol 7, Issue 1
Abstract
Efficient protocols of Guadua angustifolia Kunth micropropagation are not reported due to two principal problems for establishing it: explants necrosis and microbial contamination. In this work a high frequent bacterial contaminant in the in vitro establishment of Guadua was isolated and identified . Besides, the minimal inhibitory concentration (MIC) of Gentamicin sulphate was determined by two methods: dillution and diffusion in Agar methods. Bacterial contaminant isolated was identified as belonging to Bacillus genus. Sensitivity of Bacillus sp. to Gentamicin sulphate was proved and the value of MIC (1μgml -1 ) was obtained using both methods. The use of this antibiotic will be an alternative for the solution of in vitro bacterial contamination problems in Guadua angustifolia tissue culture once no existance of phytotoxicity is verified.
Authors and Affiliations
Mileidy Cruz-Martín, Yudith García-Ramírez, Cynthia Sánchez-García, Yelenys Alvarado-Capó, Mayra Acosta- Suárez, Berkis Roque, Michel Leiva-Mora, Marisol Freire-Seijo
Keywords
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