Immunogenicity, antigenicity and epitope mapping of Salmonella InvH protein: An in silico study
Journal Title: Journal of Current Biomedical Reports - Year 2020, Vol 1, Issue 1
Abstract
InvH is an indispensable part of T3SS-I and has a significant role in SPI-I mediated effector protein translocation. The InvH mutations have significant effects including reduced secretory and inflammatory responses that result from preventing the normal secretion of several proteins. Our team previous studies showed the capable ability of InvH to induce the humoral immune system to prevent almost all Salmonella strains infections. The current study aimed to determine all aspects of this protein using several bioinformatics tools and find the differences among all Salmonella strains. This data could pave the way for further studies about InvH protein and the production of an effective vaccine against Salmonella infections. InvH sequences for all Salmonella strains were obtained from GenBank and analyzed to determine physicochemical properties, B-Cell and T-Cell epitopes, and reliable structures. Results showed some minimal differences among Salmonella strains. B-Cell and T-Cell epitopes predicted by numerous software approved the ability of this protein to induce both humoral and cellular immune systems remarkably. This study provided a comprehensive data to determine all features of InvH protein and our results showed the ability of this protein to design a capable vaccine and the effect of amino acid changes on structure and physico-chemical properties, and epitopes.
Authors and Affiliations
Behzad Dehghani, Tayebeh Hashempour, Zahra Hasanshahi, Iraj Rasooli
Keywords
Related Articles
- EP ID EP688941
- DOI -
- Views 333
- Downloads 0
COVID-19 and respiratory tract viral co-infections: Choosing the screening method
No Abstract.
The effect of non-surgical periodontal therapy on glycemic control and C1-reactive protein levels among type 2 diabetic patients with periodontitis: A clinical trial
It suggests that non-surgical periodontal therapy without systemic antibiotic therapy may not achieve a significant improvement in glycemic control and systemic inflammation in diabetic patients. However, it is important...
Using decoction of some vitamin C enriched plants for the management of COVID-19 in Jos, Nigeria: A case report
The worldwide influence of coronavirus disease 2019 (COVID-19) pandemic compelled the global health organizations to prioritize the situation over all other related community health issues. The lack of specific treatment...
Molecular investigation of extended-spectrum β-lactamases (ESBLs) genes in the Salmonella isolates obtained from children with acute diarrhea
Salmonellosis is an important public health concern among children in worldwide. Extended-spectrum β-lactams (ESBLs) cause resistance to clinically important beta-lactams which are generally used to treat invasive Salmon...
In silico prediction of B cell epitopes of the hemolysis-associated protein 1 for vaccine design against leptospirosis
Leptospirosis is known as a zoonotic disease of global importance originated from infection with the spirochete bacterium Leptospira. Although several leptospirosis vaccines have been tested, the vaccination is relativel...