IN VITRO EVALUATION OF CYTOTOXIC AND GENOTOXIC EFFECTS OF PLANT EXTRACTS FROM NOTHAPODYTES FOETIDA (WIGHT) SLEUMER (FAMILY: ICACINACEAE)
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2018, Vol 11, Issue 12
Abstract
Objective: The objective of this study is to investigate cytotoxic and genotoxic properties of aqueous and methanolic extracts of the aerial parts of Nothapodytes foetida (Wight) Sleumer plant.Methods: The cytotoxic effects of aqueous and methanol extract of leaves and stem bark on cell viability of HeLa, MCF7, and HCT-15 cell lines was determined by 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazoliumbromide assay. We also confirmed the genotoxic effects of plant extracts through DNA fragmentation in cancer cells and expression pattern of apoptotic genes including p53 and caspase-3 analyzed by semiquantitative reverse transcription-polymerase chain reaction and western blotting techniques.Results: The present study revealed that, when plant extract was tested for cytotoxic activity, the data obtained from cell viability results of HeLa and MCF7 cells revealed that methanol extract of leaves and stem bark exhibited a range of significant cytotoxic activities in a dose-dependent manner varying from 2.5 to 25 μg/mL, whereas an aqueous extract of leaves and stem bark showed decreased cell viability with an increase in the concentration of both the extracts from 5 to 50 μg/mL.Conclusion: These results indicated that the crude extract aerial parts of N. foetida plant contain promising substances having a potential as cytotoxic agent.
Authors and Affiliations
Kailas D Datkhile, Pratik P Durgawale, Madhavi N Patil
Keywords
Related Articles
- EP ID EP605210
- DOI 10.22159/ajpcr.2018.v11i12.28087
- Views 112
- Downloads 0
UV-ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF DAPOXETINE HYDROCHLORIDE AND SILDENAFIL CITRATE IN TABLET DOSAGE FORM
Objective: The objective of this research was to develop and validate a simple ultraviolet (UV) spectrophotometric method for simultaneous determination of sildenafil citrate and dapoxetine hydrochloride in a pharmaceuti...
FORMULATION AND EVALUATION OF MICROEMULSION GEL FOR TRANSDERMAL DELIVERY OF TRAMADOL
Objective: The present work was carried out to design microemulsion gel system for transdermal delivery of the drug to minimize the side effects and to reduce the frequency of administration and for prolonging the durati...
HERBAL ACTIVE INGREDIENTS USED IN SKIN COSMETICS
Human beings are obsessed with looking beautiful. Hence, various beauty products have been in use to look charming and young. Herbal ingredients are popular worldwide for use in cosmetics. The demand for herbal cosmetic...
LEVEL OF KNOWLEDGE REGARDING HUMAN IMMUNODEFICIENCY VIRUS/ACQUIRED IMMUNE DEFICIENCY SYNDROME AND PEOPLE LIVING WITH HUMAN IMMUNODEFICIENCY VIRUS AMONG HEALTH-CARE STUDENTS OF SELECTED COLLEGES IN CHENNAI
Objective:  The  aim  of  the  study  was  to  assess  the  level  of  knowledge  among  health-care  students  (HCS)  toward  human  immunodeficiency virus/acquired immune deficiency syndrome (HIV/AID...
EFFECT OF NON-SURGICAL THERAPY ON SALIVARY NITRIC OXIDE AND LIPID PEROXIDATION LEVELS IN TYPE II DIABETIC AND NON DIABETIC PATIENTS WITH PERIODONTAL DISEASE
Objective: Reactive oxygen species have been identified as potential factors causing periodontal tissue destruction. Elevated levels of these in patients with chronic periodontitis and diabetes may aggravate the oxidativ...