Inhibitory Effect of Vernonia amygdalina on Dimethylnitrosamine (DMN)-induced Liver Fibrosis in Rats
Journal Title: International Journal of Clinical Pharmacology & Toxicology(IJCPT) - Year 2015, Vol 4, Issue 4
Abstract
In this study, the protective effect of Vernonia amygdalina ethanolic leaf extract against hepatic fibrosis induced by repeated intermittent administration of Dimethylnitrosamine (DMN) was investigated in rats. A total of 48 rats divided into 4 groups were used. Group 1 served as control, Group 2 received 200mg/kg Vernonia amygdalina only for 14days, Group 3 received 200mg/kg Vernonia amygdalina for 14 days followed by intraperitoneal administration of 10mg/kg DMN on first three days of each week for 2 weeks, while Group 4 received intraperitoneal administration of 10mg/kg DMN on the first three days of each week for 2 weeks. Oral administration of Vernonia amygdalina led to recovery of body/liver weight losses and significantly reversed elevation of total collagen, AST, ALT and ALP in DMN-exposed rats. Severe oxidative stress induced in fibrotic rats was evidenced by elevation in MDA levels associated with a fall in the activities of GSH, SOD and CAT in the DMN-treated group. These were significantly reversed by simultaneous treatment with Vernonia amygdalina. Recovery of rat liver tissue from DMN-induced hepatocellular necrosis and hepatic fibrosis by Vernonia amygdalina treatment was confirmed by histopathological evaluation of liver tissue. In conclusion, Vernonia amygdalina exhibits hepatoprotective, antioxidant, free radical-scavenging, and anti-fibrotic activity against DMN-induced hepatic fibrosis
Authors and Affiliations
Usunobun Usunomena
Keywords
Related Articles
- EP ID EP201781
- DOI 10.19070/2167-910X-1500030
- Views 90
- Downloads 0
Deterioration of Brain Cell Function Induced by Dapoxetine Administration in Male Rats
Background and Aim of Work: Dapoxetine is a Selective Serotonin Reuptake Inhibitor (SSRI) decided for the remedy of premature ejaculation (PE) in adult men. The objective of this research was to determine the influence o...
Strategies for the use of SPE
Strategies for the use of SPE
Decomposition of Concentration-Time Profiles after Oral Administration of Different Ethanol Doses in Humans
A new physiologically-motivated structural model capable of evaluating ethanol behavior within the whole-body blood metabolism was developed. This model was estimated on the basis of a reanalysis of the mean blood ethano...
Evaluation of Leukocyte DNA Damage and Antioxidant Defense in Graves’ Disease; Effect of Medical Treatment
Background: Oxidative stress has been implicated in many pathological conditions, including hyperthyroidism. In this study, our aim was to investigate the effect of medical treatment on oxidative/antioxidative status and...
Population Pharmacokinetic Analysis of Alcohol Concentration after Beer Consumption in Fasted Japanese Subjects
The absorption rate of alcohol is one of the determinants of the peak blood alcohol concentration. Studies focusing on factors that cause inter-individual variability in absorption rate have not been conducted. To conduc...