INVESTIGATION OF SEED GERMINATION IN THE TRANSGENIC CANOLA (BRASSICA NAPUS L.) UNDER SALT STRESS
Journal Title: International Research Journal of Applied and Basic Sciences - Year 2011, Vol 2, Issue 9
Abstract
Abiotic stresses such as drought and salinity are the most considerable cause of crop productivity decrease in agriculture. Genetic engineering can improve crop tolerance to abiotic stresses by many ways. Mannitol is one of plant osmoprotectants to cope with osmotic stress. In the previous study, we developed transgenic Canola (Brassica napus L.) using bacterial mtlD gene. Here, salt stress tolerance of transgenic (+mtlD) and non-transgenic (-mtlD canola plants studied on seed germination stages. The sterilized transgenic and non-transgenic seeds were cultured on MS media (pH 5.8) at different concentrations of NaCl (0 250 300, 350, 400 and 450 mM). HPLC analysis results showed that transgenic plants accumulate mannitol on average of 0.0440 mg/gDW, whereas wild-type plants did not accumulate any mannitol. However, transgenic seeds germinated at 350 mM NaCl, non-transgenic plants germinated not more than 250 mM of NaCl. Therefore, we demonstrated that the mtlD gene was expressed in transgeniccanola plants, resulting in mannitol accumulationand improved salt tolerance in transgenic seeds.
Authors and Affiliations
ROYA MOTALLEBI| Tissue Culture and Gene Transformation Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Mahdasht Road, P. O. Box 31535-1897, Karaj, Iran, HASSAN RAHNAMA*| Tissue Culture and Gene Transformation Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Mahdasht Road, P. O. Box 31535-1897, Karaj, Iran
Keywords
Related Articles
- EP ID EP4852
- DOI -
- Views 1013
- Downloads 40
The study of green consumers’ characteristics and available green sectors in the market
Attention to environment has become a key element in political decisions and various organizations and associations have been formed along with a variety of laws and regulations for environmental protection. On the other...
Principles of disinfecting domestic wastewater for irrigation
In order to remove pathogens from wastewater to be used in agriculture, World Health Organization (WHO) has published a guide book titled “Guidelines for the safe use of wastewater excreta and gray water” in four volumes...
The Effect of Emailing on Motivation of Adult English Learner of Iran
This paper originally was motivated by the claim that using emailing in language institutes has strong effect on motivation of adult learners. It investigated the motivation orientation of the Iranian English learners to...
The Investigation of the Relationship between Discretionary Accruals, Growth, and Future Firm Performance in Listed Firms at Tehran Stock Exchange (TSE)
In this research, an analysis on the relationship between Accruals, Sale Growth and future performance in Listed Firms at TSE was conducted. The purpose of this research is to study the relationship between Accruals and...
Vibration analysis of piezoelectric nanowires using the finite element method
A finite element model for free vibrations of piezoelectric nanowires (NWs) is developed based on the non-local Euler-Bernoulli beam theory and Galerkin method. The governing equation of motion for the piezoelectric NW w...