Isolation and identification of microorganisms and antibacterial activity of Laban Zeer, an Egyptian traditional fermented milk product
Journal Title: Scientific Journal of Microbiology - Year 2013, Vol 2, Issue 2
Abstract
Laban Zeer is a traditional Egyptian fermented milk product. The microorganisms of Laban Zeer were isolated and identified to species level, as well as the antibacterial activity of Laban Zeer was also studied against pathogenic bacteria. Total viable microorganisms, including, lactic acid bacteria (LAB), aerobic mesophilic bacterial, Enterococus and Enterobacteriaceae were enumerated. A total forty eight LAB and twenty eight yeast isolates were isolated from four Laban Zeer samples and identified by API 50 CHL and API 20C AUX identification system, respectively. The avenger of LAB counts were 7.4 cfu/g, while yeast and Enterococus counts were 4.67 and 4.39 cfu/g, respectively. It is noted that the count of bacteria belonging to the family of Enterobacteriaceae was not detected in all tested samples. The LAB species were identified as Leuconostoc mesenteroides subsp. cremoris, Lb. rhamnosus, Lb. plantarum, Lb. paracasei subsp paracasei, Lb. delbercii subsp bulgaricus, Lb. curvatus subsp curvatus and Lb acidophilus. The isolated yeasts were identified as Sccharomyces cervisiae, Candida kefyr, Candida utilis and Rhodotorula mucilaginosa. The most frequently isolated species was found to be Leuconostoc mesenteroides subsp. cremoris (37.5%), Lb. rhamnosus (20.8%), Sccharomyces cervisiae (41.9%) and Candida kefyr (29.0%). The antimicrobial activities of Laban Zeer were evaluated in vitro using an agar well diffusion method and in situ method. The major supernatants of Laban Zeer samples inhibited the growth of pathogenic bacteria, belonging to Escherichia, Pseudomonas, Salmonella, Listeria and Staphylococcus genera in various degrees. The in situ method was performed by the inoculation of Staph. aureus and E. coli in Laban Zeer samples separately at an initial level around of 6 log cfu/ml. The count of Staph. aureus and E. coli were not detected after 12 and 3 days of refrigerated storage period, respectively in samples number 2 and 3. Laban Zeer is not suitable environment for growth and activity of such pathogen and would eliminate the pathogen early enough before the products were made ready for consumption. The microorganisms isolated from Laban Zeer can be used widely in the food fermentation industry as starter culture and bio-preservatives due to their broad inhibition spectrum.
Authors and Affiliations
F. A. Saleh| Food and Nutrition Sciences Dep. College of Agriculture and Food Science, King Faisal University, Saudi Arabia.
Keywords
Related Articles
- EP ID EP1160
- DOI -
- Views 531
- Downloads 30
Distribution of Aspergillus species among apparently healthy birds in poultry farms in Kaduna state, Nigeria
The study wasconducted to determine the prevalence and distribution of Aspergilllus species among apparently healthy birds in poultryfarms. Trachea swabs from 1500 birds in 52 commercial (10% of birds in eachpoultry farm...
Evaluation of the effectiveness of bleach on microbial population of lettuce
The effectiveness of bleach on the microbial population of lettuce was evaluated. Microorganisms on lettuce before and after washing with bleach were searched and counted and a new dose for cleaning to eliminate a high c...
In-vitro antibacterial evaluation of ethanolic stem crude extracts of anacardium occidentale linn. (anacardiaceae) on streptococcus mutans associated with dental caries
The preliminary phytochemistry and antibacterial activity of ethanolic stem crude extracts of Anacardium occidentale on Streptococcus mutans isolated from dental caries were determined using chemical, standard microbiolo...
Characterization and antibiotic susceptibility pattern of Bacillus cereus isolates from fried soyabean cake in Zaria, Nigeria
A total of one hundred and fifty fried soyabean cake samples were collected from different vendors in various parts of Zaria, Kaduna state and were assessed for the presence of Bacillus cereus. 84 out of the total 150 we...
Prevalence and evolution of intestinal parasites and urinary in hospital luxembourg: endemicity risk of ascaris lumbricoides and entamoeba histolytica in the district of bamako
The aim of the study was to determine the prevalence and evolution of intestinalis parasites in hospital area. For the present investigation, 2401 samples, including 403 urines and 1998 stool, were collected. The age of...