Isolation, Characterization and Antibiotic Susceptibility of Mycoplasma hominis and Ureaplasma urealyticum from Infertile and Pregnant Women in Lagos, Nigeria
Journal Title: Microbiology Research Journal International - Year 2017, Vol 20, Issue 1
Abstract
Background: Mycoplasma hominis and Ureaplasma urealyticum are potentially pathogenic agents, playing aetiologicroles in both genital infections and infertility. In human in-vitro fertilization systems, the presence of U. urealyticum in either semen or female genital tract results in a decline of pregnancy rate per embryo transfer as well as neonatal infections. M. hominis has been associated with bacterial vaginosis, pelvic inflammatory disease, postabortal fever, and a number of gynecological infections. Aim: The aim of this study is to isolate, characterize and determine the antibiotic susceptibility of M. hominis and U. urealyticum isolates from infertile and pregnant women in Lagos, Nigeria. Materials and Methods: The samples were collected from Obstetrics and Gynaecology clinics of Lagos University Teaching Hospital and 68 Nigerian Army reference hospital Yaba. A total of 270 specimens of urine and HVS were collected from 135 women attending the clinics for routine consultations. One hundred and eighty HVS and urine specimens were from 90 married infertile women attending the clinics as part of a work-up for fertility investigations after failing to conceive for at least one year of unprotected sexual intercourse. Ninety HVS and urine specimens were from 45 pregnant women attending the clinics for routine antenatal care. None of the subjects expressed any symptom of genitourinary tract infections. All the specimens were inoculated into Mycoplasma broth and subsequently Blood Agar plates, incubated appropriately and identified. Antibiotic susceptibility tests were carried out on the 52 isolates. Polymerase chain reaction (PCR) was used to detect the organisms in all the collected specimens. Results: Of the 90 HVS specimens collected from infertile women, 9 (10.0%) were positive for M. hominis, while 21 (23.3%) were positive for U. urealyticum. For the pregnant women using HVS specimens, 6 (13.3%) were positive for M. hominis while 5 (11.1%) were positive for U. urealyticum. The first void urine specimens gave lower values in both the infertile and pregnant women. Prevalence of U. urealyticum was higher in infertile women than in pregnant women (p<0.05). The PCR technique gave higher values of 78.5% and 71.1% using HVS specimens for the infertile and pregnant women respectively for Mycoplasma/Urealyticum species. The antibiotic susceptibility test showed that all the isolates of M. hominis (n=18) were sensitive to Tetracycline (100%) and Ciprofloxacin (100%) while all the isolates of U. urealyticum (n=34) were sensitive to Tetracycline (100%) and Erythromycin (100%). Conclusion: The significantly higher prevalence of U. urealyticum infection in infertile women (23.3%) compared to the lower prevalence in pregnant women (11.1%) may suggest that U. urealyticum can be incriminated in infertility. HVS specimen is preferred over urine specimens for the detection of Mycoplasma and Ureaplasma. Application of the PCR method, where affordable, is recommended for rapid and sensitive detection of Mycoplasma and Ureaplasma in HVS specimens. Tetracycline may be the antibiotic of choice, unless contraindicated, for the treatment of the infections, although the sample size was small.
Authors and Affiliations
Eugene Ikeh, Mike Ebie, John Allanana, Nneoma Idika
Keywords
Related Articles
- EP ID EP315949
- DOI 10.9734/MRJI/2017/33323
- Views 101
- Downloads 0
Isolation and Characterization of a Marine Bacterium from Sundarbans, Bangladesh
Aims: Marine environment is a vast but represents a largely untapped source for isolation of new microorganism having ability to produce novel bioactive compound(s). The present study was designed with an aim to discover...
Effect of Selected Conditions on Spore Populations Outgrowth Dynamics and Time to Single Spore Outgrowth Distribution in Bacillus subtilis and Bacillus cereus Spores Population
Aims: In this study the effect of sporulation conditions, namely pH, CaCl2 concentration and the type of spore activation was assessed on the population outgrowth kinetics parameters and time to individual spore outgrowt...
Suppression of Phytopathogenic Fungi by Plant Extract of Some Weeds and the Possible Mode of Action
Aims: The goal of this work was to emulate the antifungal properties of some newly collected weeds against important phytopathogenic fungi: Pythium ultimum, Penicillium expansum and Fusarium solani. The mode of action th...
Bacteriological and Physico-chemical Analysisof Borehole Water in Auta Balefi Community, Nasarawa State, Nigeria
Aim: To determine the bacteriological and physico-chemical qualities of borehole water in Auta Balefi, community. Place and Duration of Study: Auta Balefi, Community, Karu LGA, Nasarawa State; Department of Biological Sc...
Occurrence of Enteric Pathogens on Fresh Produce Grown on Irrigated Soils
Aims: To assess the potential health risks of fresh produce grown on irrigated soils treated with manure in Kano State, a large produce region in Nigeria. Methodology: Fresh produce irrigated with wastewater on manure t...