METHOD DEVELOPMENT AND VALIDATION OF RP-HPLC ANALYSIS OF BROMFENAC IN RABBIT PLASMA
Journal Title: Int J of Pharm Res & Analy - Year 2014, Vol 4, Issue 8
Abstract
A simple, precise, rapid, and accurate reverse phase high performance liquid chromatography method was developed for the estimation of Bromfenac in Rabbit plasma by using Paracetamol as IS. The protein precipitation technique was used for the extraction of analyte from rabbit plasma. The chromatographic separation was performed on Agilent, Zorbas SB C18 (4.6mm×250mm×5μ) column with PDA detection at 267nm. The mobile phase comprising of mixture of methanol and buffer 0.1% ortho phosphoric acid (pH 2.6) in the ratio of 30:70v/v, at the flow rate 0.8ml/min. The analytical method described here is valid for the estimation of Bromfenac in Rabbit plasma over a range of 50μg/ml-150μg/ml. The retention times of Bromfenac and Paracetamol were found to be 3.781 and 2.470mins respectively. The correlation coefficient for Paracetamol and Bromfenac were 0.998 and 0.999 respectively. The LOD and LOQ values for Bromfenac were found to be 0.13μg/ml and 0.40μg/ml respectively. %RSD of Bromfenac and Paracetamol was obtained to be 0.41and 0.28 respectively. This method is time efficient and samples are easy to monitoring Bromfenac in plasma.
Authors and Affiliations
Vidya Kamdar , Uma Maheshwara Rao , Vattikuti , Ajitha Azhakesan
Keywords
Related Articles
- EP ID EP112008
- DOI -
- Views 135
- Downloads 0
SCREENING OF WOUND HEALING ACTIVITY OF BARK OF ALEURITES MOLUCCANA L.
Aleurites moluccana L. is used in folklore medicine in fomenting, sealing of secondary infection, healing of wounds and skin eruptions. There was no scientific evidence justifying the use of bark of Aleurites moluc...
A REVIEW ON ANALYTICAL METHOD DEVELOPMENT AND VALIDATION
Analytical methods development must be validated to provide reliable data for regulatory submissions. Method development is the process of proving that an analytical method is acceptable for use to measure the concentrat...
FORMULATION AND EVALUATION OF ORAL THERMOREVESIBLE IN SITU GEL CONTAINING FLUCONAZOLE
The objective of this study was to formulate and evaluate in situ oral topical gels of Fluconazole based temperature induced systems for the treatment of oral condidiasis. Conventional oral formulations like...
MICROSPONGES: A NOVEL DRUG DELIVERY SYSTEM FOR CONTROLLED DELIVERY OF TOPICAL DRUGS
Even though possess several advantages topical formulations have some drawbacks such as the need to contain high concentrations of active agents for effective therapy due to the low efficiency of delivery system, r...
SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF Fe(II) AND Zn(II) IN MULTIVITAMIN SOFT GEL CAPSULE
The present work describes novel spectrophotometric method for the determination of Fe(II) and Zn(II) in multivitamin soft gel formulation. The method is based on formation of coloured complexes of Fe(II) and Zn(II...