Molecular and pathological studies of duck hepatitis virus in Egypt
Journal Title: Journal of Veterinary Medical Research - Year 2017, Vol 24, Issue 2
Abstract
Duck Hepatitis virus (DHV) causes great economic losses in waterfowl industry worldwide. 3D gene, lies in the P3 segment of the picornavirus genome, is highly conserved and is a non-structural polyprotein gene, its encoding protein contains a conserved domain termed RNA-dependentRNA polymerase which participates in the synthesis of virus RNA during virus replication. The current work describes the surveillance of DHV in 20 commercial duck farms in Egypt with a history of high mortality in 3 to 15 day-old young ducklings from 2014 to 2016. Clinical samples were examined by Real Time-Polymerase Chain Reaction assays followed by partial sequence analysis of the 3D gene of the positive samples. Histopathological examination of the liver from selected samples was also conducted. The overall positive rate was 50% (n = 10/20). All duck breeds (Pekin, Muscovy and Mallard) used in the study were found to be susceptible to the disease. Histopathological findings of the liver samples showed pronounced lesions including hepatocyte degeneration and necrosis. Also, apoptosis were observed and bile duct hyperplasia, together with varying degrees of inflammatory cell response and haemorrhage. Sequence and phylogenetic analyses indicated that the Egyptian strains cluster in the DHAV serotype 1 with Asian viruses and distant from the vaccine strains used in Egypt.
Authors and Affiliations
Ali Zanaty, Naglaa Hagag, Mohamed Samy, Ahmed abdel-Halim, Abdel-Satar Arafa, Soad Nasif
Keywords
Related Articles
- EP ID EP658362
- DOI -
- Views 236
- Downloads 0
Risk assessment of some residues in milk and some dairy products
Milk and dairy products are an important exposure route for organochlorine pesticides (OCPs) to humans. For this reason milk, kareish cheese and yoghurt analyzed for the presence of fourteen organochlorine pesticides nam...
Fungal pathogens associated with respiratory problems in broiler chickens
Fungal diseases of poultry have become problematic as bacterial and viral diseases. Therefore, this study was designed to investigate the prevalence of fungal agents in broiler chickens suffering from respiratory disorde...
Field evaluation of inactivated Corynebacterium ovis vaccine in sheep prepared from a local isolate
Corynebacterium pseudotuberculosis vaccine was prepared from a local field isolate. Vaccination of sheep with 50g PLD toxoid and 10 mg bacterin adjuvanted with Montanide oil improved the levels of immune responses of sh...
Studies on the effect of different immunostimulants on chick's immune response to inactivated avian influenza and Newcastle Vaccines
Newcastle Disease virus (NDV) and Avian Influenza virus (AI) are represent a great negative significant causing severe economic losses and increased mortalities worldwide. Newcastle disease (ND) and Avian Influenza (AI)...
Effect of seasonal variations on performance and parasitic infestation of cultured fish in Fayoum governorate
A field study was carried out in a fish farm to study the effect of seasonal variations during the production period on performance and parasitic infestation of cultured fish. Water samples were obtained to determine the...