Molecular based test for detection of cyanotoxins in the domestic drinking water tanks in Baghdad
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2018, Vol 13, Issue 1
Abstract
This study was conducted to assess and analyze the common cyanobacteria in the domestic drinking water tanks in Baghdad and to see if they are potentially toxigenic.The study included isolating toxic blue green algae from domestic plastic water tanks .Samples were collected during September 2016 to March 2017. The microscopic diagnosis of the samples examined showed that the predominant blue-green algae which were (Synechococcus elongatus sp., Lyngbya sp.1, Anabena aequalis, Lyngbya sp.2).In this study, four sets of primers were used to detect the genetic material of blue-green algae and detect the toxins of mycrostin, cylindrospermopsin and saxitoxin. Molecular analysis showed that all isolates contained phycocyanin that shared by all cyanobacteria. The mycE gene was not detected in all isolated cyanobacteria and blooming samples, while the sxtA and aoaC genes were detected in isolated cyanobacteria and not detected in blooming samples. The aim of this study was to detect the cyanotoxin by using PCR. PCR was found to be a reliable and rapid method to test for the presence of potentially toxigenic cyanobacteria as early warning system to predict toxic cyanobacterial bloom in the domestic drinking water tanks in Baghdad
Authors and Affiliations
Ibrahim J. Abed, 1Raneen N. Musbah
Keywords
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- EP ID EP393204
- DOI 10.9790/3008-1301017681
- Views 93
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