Optimization of Nutrient Components for Tacrolimus Production by Streptomyces tsukubaensis using Plackett-Burman followed by Response Surface Methodology
Journal Title: IOSR Journal of Pharmacy (IOSRPHR) - Year 2014, Vol 4, Issue 7
Abstract
Tacrolimus belongs to macrolide lactone groups isolated from Streptomyces tsukubaensis, is used to prevent organ transplantation rejection. The aim of this study was to enhance the production of Tacrolimus by optimizing the nutrient components in fermentation media. Nutrient components play a significant role in the production of secondary metabolites. Many optimization techniques are available for this study and each optimization techniques has its own advantages. Plackett-Burman multifactorial design was employed to evaluate the significant nutrient factor in the medium using high and low levels of the factors where Dextrine white, Cotton seed meal (CSM) and Polyethylene glycol (PEG)-400 were found most important components among eight variables. These screened components were further optimized by central composite design with response surface methodology. A central composite design was based on second order polynomial used to determine the optimal levels of screened variables. The optimal level of each variable obtained from polynomial model was 173.94 g/l dextrine white, 19.83 g/l Cotton seed meal (CSM) and 24.72 g/l Polyethylene glycol (PEG)-400 respectively. Tacrolimus yield i.e., 1.08 mg/g was achieved with the combination of these optimal values. Validation experiments were performed to verify the adequacy and accuracy of the model
Authors and Affiliations
Archana R. Tripathi, Nishtha K. Singh, Umesh luhtra
Keywords
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