RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF AMLODIPINE BESYLATE AND TELMISARTAN IN TABLET DOSAGE FORM
Journal Title: Int J of Preclin & Pharm Res - Year 2013, Vol 3, Issue 1
Abstract
ABSTRACT A simple, sensitive and rapid reverse phase HPLC method was developed for the simultaneous estimation of Amlodipine besylate and Telmisartan. A Phenominex-luna C18 column (250x4.6 mm i.d 5µ) was used with a mobile phase containing a mixture of acetonitrile and phosphate buffer in the ratio of 56:44%v/v.pH was adjusted with orthophosphoric acid to 4.The flow rate was 1ml/min and the eluents were monitored at the detector wavelength of 236nm.The retention times of Amlodipine besylate and Telmisartan were found to be 4.32 and 5.32 minutes respectively.The validation of the proposed method was carried out for its specificity, accuracy, pecision, linearity, limit of detection and limit of quantification for both Amlodipine and Telmisartan. Keywords: Amlodipine besylate, Telmisartan, Reverse phase HPLC.
Authors and Affiliations
Rajeswari A , Vasanth PM* , Ramesh T , Ramesh Malothu
Keywords
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