Optimization of extraction process and investigation of Antioxidant Activity , DNA Protection Potential and Antimicrobial activities of Trachyspermum ammi seed extract
Journal Title: IOSR Journal of Applied Chemistry (IOSR-JAC) - Year 2018, Vol 11, Issue 11
Abstract
The free radicals are produced by various metabolic processes in our body. In excess these free radicals can cause damage to important cellular organs. Antioxidants are substances which can prevent this damage by quenching free radicals. Antioxidants of plant origin are in demand due to less side effects. The plant Trachyspermum ammi (Ajwain) is used to cure many diseases and possess antioxidant and antimicrobial activities. The present research is an attempt to optimize extraction process to enhance antioxidant and antimicrobial properties of seed extract of Ajwain. The methanolic extracts of seeds were prepared by cold , hot and Soxhalate method. Folin-Ciocalteu spectrophotometric method was used to estimate TPC. The antioxidant activity was evaluated by DPPH method. The DNA damage was done using super coiled pBR322 plasmid and antimicrobial activity was assessed by well diffusion method. The results showed all extracts possess antioxidant activity and have ability to prevent DNA damage. The positive antimicrobial property was shown by extract obtained from cold extraction method. The cold extraction method was found to be more effective in enhancing medicinal property of extract.
Authors and Affiliations
Aqeela A. S. Qureshi, Gayatri Barabde
Keywords
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- EP ID EP422631
- DOI 10.9790/5736-1111014549.
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