Partial purification of antibiotic compound from resistant bacteria species isolated from Bhavani river water
Journal Title: International Journal of Pharmaceutical and Medicinal Reserach - Year 2016, Vol 4, Issue 3
Abstract
The river Bhavani is the second largest river in Tamil Nadu, South India and a major tributary of the Cauvery River. The chemical manufactures, sugar mills, textile units, tanneries, paper industries, etc., discharges the treated and untreated wastes into the Bhavani river. The study reveals collection from various sites of Bhavani River, Mettur, Pallipalayam and Kumarapalayam. The microbes were approached by culture-dependent for understanding the physiological, morphological and biological property. Pure colony isolation and analysis of sensitivity by disc diffusion method were done. As the uptake of toxic substances by the microorganisms for its growth, so they are possessing high resistivity. Among 26 isolates 19 isolates showed maximum sensitivity (10 mm - 32 mm) and 8 isolates showed moderate sensitivity (1 mm - 10 mm) to different antibiotic disk. Among all the isolates, isolate 19 showed maximum resistances to all antibiotics (≥ 4mm). Hence the isolate 19 was further purified by column chromatography, fraction 17 showed antimicrobial activities to 5 microorganisms.
Authors and Affiliations
A. Saravanan, P. R. Yaashikaa, S. Divya, R. Keerthana, S. Shanmathi
Keywords
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