Phytochemical, Antibacterial and Antioxidant Activity of Camellia Sinensis Methanolic and Aqueous Extracts
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2016, Vol 11, Issue 6
Abstract
This study evaluated the antioxidant and antibacterial activity of green tea by using the methanolic and aqueous extract. phytochemical analysis also detected. Free radical scavenging activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl(DPPH) free radical. The methanolic crude extracts of green tea (Camellia sinensis) was the strongest, The EC50 of the methanolic extracts was 2.9 μg/ml and that of ascorbic acid was 2.9 μg/ml. Followed by BHT with value 4.0 μg/ml while The EC50 of the aqueous extracts was 6.5 μg/ml that showed less free radical scavenging activity with the DPPH method. The study reveals that the consumption of green tea has several beneficial effects by virtue of their antioxidant activity.Phytochemical analysis revealed the presence flavonoids, alkaloid, tannins and absence of glycosides, terpens and saponins. Camellia sinensis can inhibit the growth of Gram-positive and Gram-negative bacterial species with moderate potency. The study was carried out on E coli and Staphylococcus aureus, Pseudomonas aeriginosa and Bacillus subtilus and shows that the methanolic extract have better inhibitory effect than aqueous one in a concentration (40 mg/ml) against Escherichia coli and Staphylococcus aureus, the diameter of the inhibition zones was (15and 16 mm) respectively and show a lesser extent to Bacillus subtilus and Pseudomonas aeriginosa.
Authors and Affiliations
Noor S. Latteef
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